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5 Steps to Reduce the Length of the Research Paper Without Losing Content

5 steps to reduce the length of the research paper

You have reached the point where you are happy with your research, you’ve completed a manuscript and selected a target journal. Yet while doing the final checks, you find that one of those pesky requirements sets the maximum length of the research paper, which you have greatly exceeded.

As an editor and journal reviewer, I often find that authors use too many words to convey one idea, either because they have too much to say or English is not their first language. This redundancy occurs when you unnecessarily repeat something that can muddle the message and divert the reader’s attention, which should be avoided. But, how to shorten a research paper while making sure your message is clear? Do not despair, I have five simple tips to help you achieve just that.

1. The first thing to reduce word count in research papers is to focus. Concepts should be defined once, either in the Introduction or Discussion section, but not both. Methodologies are also only needed once, and if established then make sure to cite the relevant reference. Use tables and figures to present the data; numbers from tables or figures should not be repeated in the text except in unusual cases where the numbers have a special significance. The conclusion should summarize the answers to the research question and not repeat the results.

2. Avoid redundancy. What do I mean by redundant? It refers to unnecessary words. For example, “all over the world” can be replaced with “worldwide” and instead of writing “in spite of the fact that” you can use “although.” Redundancy also happens when using two words together that have the same meaning (e.g., merge together, close proximity, end result, or shorter/longer in length). Another way to reduce the length of a research paper is to avoid repetition in your sentences (e.g., for the measurement of the nitrogen concentration, we measured the content of nitrogen using the Kjeldahl method; everything before the comma can be deleted). Hyphenated words are usually considered one word, so you can also hyphenate compound adjectives that modify a noun (e.g., water-soluble fertilizers instead of fertilizers that are soluble in water). When giving a comparison for which there are an equal number of elements, use the word “respectively” (e.g., the oxygen and nitrogen flow were set at 80 and 5 ml/min, respectively).

how to make a research paper shorter

3. When citing papers, avoid using introductory expressions. How often do you read papers repeating “another research study found/demonstrated that” or “scientists have noted that” or the sometimes inexplicable need to use the author name? State your claim directly and cite the relevant literature, your prose will be remarkably more authoritative and it will also reduce the word count in research papers.

4. Using irrelevant words is a common pitfall. Don’t use words such as “notably” or “interestingly” or “unfortunately.” it may be just one word but they are redundant in scientific writing and can be deleted to shorten your research paper as they add no value. Also, avoid superlatives––adjectives used to mean something is the best of its kind––such as “it is extremely hot.” Give details (e.g., the precise temperature) and let the numbers do the talking.

5. As some journals set a very low word limit for this section, the length of the Abstract will be the ultimate challenge. Has someone ever told you “so, what do you do?” This means we used too many words and we either lost attention or they got lost with too many details. The Abstract should be a clear standalone passage of your research. A brief rationale (e.g., while X and Y are essential to A and B, little is known) with the study aim, the overall methodological approach followed by the new information uncovered (trends, not every data obtained), and the conclusions – simply present the implications of the study findings.

The punchline is that if you do not comply with the most basic requirements, your manuscript will be rejected without review. Let’s be honest, it is not about the decoration we provide but the message we convey. So I hope these steps will help you reduce the word count in research papers.

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Related Reads:

  • Confusing Elements of a Research Paper That Trip Up Most Academics
  • 3 Easy Ways for Researchers to Improve Their Academic Vocabulary
  • Self-Editing: The Pros and Cons of Editing Your Own Research Paper
  • What is an Expository Essay and How to Write It

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Grad Coach

How To Write A Research Paper

Step-By-Step Tutorial With Examples + FREE Template

By: Derek Jansen (MBA) | Expert Reviewer: Dr Eunice Rautenbach | March 2024

For many students, crafting a strong research paper from scratch can feel like a daunting task – and rightly so! In this post, we’ll unpack what a research paper is, what it needs to do , and how to write one – in three easy steps. 🙂 

Overview: Writing A Research Paper

What (exactly) is a research paper.

  • How to write a research paper
  • Stage 1 : Topic & literature search
  • Stage 2 : Structure & outline
  • Stage 3 : Iterative writing
  • Key takeaways

Let’s start by asking the most important question, “ What is a research paper? ”.

Simply put, a research paper is a scholarly written work where the writer (that’s you!) answers a specific question (this is called a research question ) through evidence-based arguments . Evidence-based is the keyword here. In other words, a research paper is different from an essay or other writing assignments that draw from the writer’s personal opinions or experiences. With a research paper, it’s all about building your arguments based on evidence (we’ll talk more about that evidence a little later).

Now, it’s worth noting that there are many different types of research papers , including analytical papers (the type I just described), argumentative papers, and interpretative papers. Here, we’ll focus on analytical papers , as these are some of the most common – but if you’re keen to learn about other types of research papers, be sure to check out the rest of the blog .

With that basic foundation laid, let’s get down to business and look at how to write a research paper .

Research Paper Template

Overview: The 3-Stage Process

While there are, of course, many potential approaches you can take to write a research paper, there are typically three stages to the writing process. So, in this tutorial, we’ll present a straightforward three-step process that we use when working with students at Grad Coach.

These three steps are:

  • Finding a research topic and reviewing the existing literature
  • Developing a provisional structure and outline for your paper, and
  • Writing up your initial draft and then refining it iteratively

Let’s dig into each of these.

Need a helping hand?

how to make a research paper shorter

Step 1: Find a topic and review the literature

As we mentioned earlier, in a research paper, you, as the researcher, will try to answer a question . More specifically, that’s called a research question , and it sets the direction of your entire paper. What’s important to understand though is that you’ll need to answer that research question with the help of high-quality sources – for example, journal articles, government reports, case studies, and so on. We’ll circle back to this in a minute.

The first stage of the research process is deciding on what your research question will be and then reviewing the existing literature (in other words, past studies and papers) to see what they say about that specific research question. In some cases, your professor may provide you with a predetermined research question (or set of questions). However, in many cases, you’ll need to find your own research question within a certain topic area.

Finding a strong research question hinges on identifying a meaningful research gap – in other words, an area that’s lacking in existing research. There’s a lot to unpack here, so if you wanna learn more, check out the plain-language explainer video below.

Once you’ve figured out which question (or questions) you’ll attempt to answer in your research paper, you’ll need to do a deep dive into the existing literature – this is called a “ literature search ”. Again, there are many ways to go about this, but your most likely starting point will be Google Scholar .

If you’re new to Google Scholar, think of it as Google for the academic world. You can start by simply entering a few different keywords that are relevant to your research question and it will then present a host of articles for you to review. What you want to pay close attention to here is the number of citations for each paper – the more citations a paper has, the more credible it is (generally speaking – there are some exceptions, of course).

how to use google scholar

Ideally, what you’re looking for are well-cited papers that are highly relevant to your topic. That said, keep in mind that citations are a cumulative metric , so older papers will often have more citations than newer papers – just because they’ve been around for longer. So, don’t fixate on this metric in isolation – relevance and recency are also very important.

Beyond Google Scholar, you’ll also definitely want to check out academic databases and aggregators such as Science Direct, PubMed, JStor and so on. These will often overlap with the results that you find in Google Scholar, but they can also reveal some hidden gems – so, be sure to check them out.

Once you’ve worked your way through all the literature, you’ll want to catalogue all this information in some sort of spreadsheet so that you can easily recall who said what, when and within what context. If you’d like, we’ve got a free literature spreadsheet that helps you do exactly that.

Don’t fixate on an article’s citation count in isolation - relevance (to your research question) and recency are also very important.

Step 2: Develop a structure and outline

With your research question pinned down and your literature digested and catalogued, it’s time to move on to planning your actual research paper .

It might sound obvious, but it’s really important to have some sort of rough outline in place before you start writing your paper. So often, we see students eagerly rushing into the writing phase, only to land up with a disjointed research paper that rambles on in multiple

Now, the secret here is to not get caught up in the fine details . Realistically, all you need at this stage is a bullet-point list that describes (in broad strokes) what you’ll discuss and in what order. It’s also useful to remember that you’re not glued to this outline – in all likelihood, you’ll chop and change some sections once you start writing, and that’s perfectly okay. What’s important is that you have some sort of roadmap in place from the start.

You need to have a rough outline in place before you start writing your paper - or you’ll end up with a disjointed research paper that rambles on.

At this stage you might be wondering, “ But how should I structure my research paper? ”. Well, there’s no one-size-fits-all solution here, but in general, a research paper will consist of a few relatively standardised components:

  • Introduction
  • Literature review
  • Methodology

Let’s take a look at each of these.

First up is the introduction section . As the name suggests, the purpose of the introduction is to set the scene for your research paper. There are usually (at least) four ingredients that go into this section – these are the background to the topic, the research problem and resultant research question , and the justification or rationale. If you’re interested, the video below unpacks the introduction section in more detail. 

The next section of your research paper will typically be your literature review . Remember all that literature you worked through earlier? Well, this is where you’ll present your interpretation of all that content . You’ll do this by writing about recent trends, developments, and arguments within the literature – but more specifically, those that are relevant to your research question . The literature review can oftentimes seem a little daunting, even to seasoned researchers, so be sure to check out our extensive collection of literature review content here .

With the introduction and lit review out of the way, the next section of your paper is the research methodology . In a nutshell, the methodology section should describe to your reader what you did (beyond just reviewing the existing literature) to answer your research question. For example, what data did you collect, how did you collect that data, how did you analyse that data and so on? For each choice, you’ll also need to justify why you chose to do it that way, and what the strengths and weaknesses of your approach were.

Now, it’s worth mentioning that for some research papers, this aspect of the project may be a lot simpler . For example, you may only need to draw on secondary sources (in other words, existing data sets). In some cases, you may just be asked to draw your conclusions from the literature search itself (in other words, there may be no data analysis at all). But, if you are required to collect and analyse data, you’ll need to pay a lot of attention to the methodology section. The video below provides an example of what the methodology section might look like.

By this stage of your paper, you will have explained what your research question is, what the existing literature has to say about that question, and how you analysed additional data to try to answer your question. So, the natural next step is to present your analysis of that data . This section is usually called the “results” or “analysis” section and this is where you’ll showcase your findings.

Depending on your school’s requirements, you may need to present and interpret the data in one section – or you might split the presentation and the interpretation into two sections. In the latter case, your “results” section will just describe the data, and the “discussion” is where you’ll interpret that data and explicitly link your analysis back to your research question. If you’re not sure which approach to take, check in with your professor or take a look at past papers to see what the norms are for your programme.

Alright – once you’ve presented and discussed your results, it’s time to wrap it up . This usually takes the form of the “ conclusion ” section. In the conclusion, you’ll need to highlight the key takeaways from your study and close the loop by explicitly answering your research question. Again, the exact requirements here will vary depending on your programme (and you may not even need a conclusion section at all) – so be sure to check with your professor if you’re unsure.

Step 3: Write and refine

Finally, it’s time to get writing. All too often though, students hit a brick wall right about here… So, how do you avoid this happening to you?

Well, there’s a lot to be said when it comes to writing a research paper (or any sort of academic piece), but we’ll share three practical tips to help you get started.

First and foremost , it’s essential to approach your writing as an iterative process. In other words, you need to start with a really messy first draft and then polish it over multiple rounds of editing. Don’t waste your time trying to write a perfect research paper in one go. Instead, take the pressure off yourself by adopting an iterative approach.

Secondly , it’s important to always lean towards critical writing , rather than descriptive writing. What does this mean? Well, at the simplest level, descriptive writing focuses on the “ what ”, while critical writing digs into the “ so what ” – in other words, the implications . If you’re not familiar with these two types of writing, don’t worry! You can find a plain-language explanation here.

Last but not least, you’ll need to get your referencing right. Specifically, you’ll need to provide credible, correctly formatted citations for the statements you make. We see students making referencing mistakes all the time and it costs them dearly. The good news is that you can easily avoid this by using a simple reference manager . If you don’t have one, check out our video about Mendeley, an easy (and free) reference management tool that you can start using today.

Recap: Key Takeaways

We’ve covered a lot of ground here. To recap, the three steps to writing a high-quality research paper are:

  • To choose a research question and review the literature
  • To plan your paper structure and draft an outline
  • To take an iterative approach to writing, focusing on critical writing and strong referencing

Remember, this is just a b ig-picture overview of the research paper development process and there’s a lot more nuance to unpack. So, be sure to grab a copy of our free research paper template to learn more about how to write a research paper.

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7 Best Ways to Shorten an Essay

7 Best Ways to Shorten an Essay

  • Smodin Editorial Team
  • Published: May 14, 2024

Are you removing a lot of words and paragraphs from your essay but still not seeing the word count budge? Whether you’re meeting a strict word count or refining your message, reducing your essay’s length without sacrificing content quality can be challenging.

Luckily, besides just aiming for the minimum word count, there are some pretty simple solutions, like using artificial intelligence, conducting thorough research, and trimming unnecessary words. But there’s more.

In this guide, we’ll unpack some practical tips to help you make your essay concise and impactful. Time to make every word count!

7 Best Ways To Shorten an Essay

Here’s a detailed breakdown of the best ways you can shorten your essay:

1. Use Artificial intelligence

When we talk about academic writing, artificial intelligence (AI) can be a game changer, especially when it comes to reducing the length of your essays.

Tools like Smodin can help make your content more concise while enhancing overall quality. AI can help you shorten your essay through the following methods:

  • Automated rewriting : AI rewriting tools can reformulate existing content to make it more straightforward while maintaining the original meaning.
  • Sentence simplification : Algorithms can analyze your sentences and suggest simpler alternatives, helping eliminate redundant information and reduce word count.
  • Research assistance : Certain platforms have AI-powered research tools that allow you to quickly gather the most relevant information. This ensures that every word in your essay contributes to your argument without unnecessary fillers.
  • Plagiarism check : Ensuring your essay is plagiarism-free is crucial. For example, Smodin’s plagiarism detection tools help you identify and replace copied content with original, concise expressions.
  • Instant feedback : Receive real-time suggestions on how to streamline your text, focusing on the essentials to effectively communicate your message.
  • Reference generation : Automatically generate and insert citations in the correct format, which helps save you time while maintaining the academic integrity of your essay and keeping it short.

2. Identify Unnecessary Words and Remove Them

One of the simplest yet most effective ways to shorten your essay is by identifying and eliminating unnecessary words.

This approach helps decrease word count and sharpens your arguments, making your writing more compelling. You can identify and remove extra words by doing the following:

  • Spot wordy phrases : Often, phrases can be condensed without losing meaning. For example, the phrase “due to the fact that” can be replaced with “because.” Be on the lookout for wordy phrases that increase word count needlessly.
  • Remove unnecessary prepositional phrases : Prepositional phrases can be redundant or add unnecessary detail. Evaluate whether these phrases add value or just extra words. Cutting them can make sentences more direct.
  • Avoid redundancies : Redundant pairs like “absolutely essential” or “future plans” can be reduced to one word without losing informational value.
  • Trim excess adjectives and adverbs : Adjectives and adverbs can make writing better but can also lead to over-description. Use them sparingly, especially when they don’t contribute additional meaning to the nouns and verbs they modify.
  • Fewer words; more impact : Aim for brevity by using fewer words to express the same idea. This will help to reduce the word count while making your writing more impactful and clear.

3. Tighten Sentence Structure

Tightening your sentence structure is crucial for making your essay more concise and readable. Use active voice to make your writing clearer and more dynamic. This is especially important in academic writing, where you have to get to the point quickly.

In academic essays, shifting from passive voice to active voice can shorten and strengthen your sentences. For example, instead of writing, “The experiment was conducted by the students,” you can say, “The students conducted the experiment.” This reduces the number of words and places the action directly with the subject, making your sentences more direct.

Combining two separate sentences into one can streamline your ideas and reduce redundancies. Look for opportunities where sentences can be merged without losing their significance. For example, “He wrote the book. It became a bestseller.” can be rephrased as “He wrote the book, which became a bestseller.”

Also, avoid unnecessary qualifiers and modifiers that don’t add substantial information. Sentences often become bogged down with these extras, making them cluttered and long.

4. Conduct Thorough Research

When writing essays, extensive research can make the final output a lot shorter. Effective research helps you gather precise information that’s relevant to your topic. This means you’ll write more directly and avoid needless elaboration. Here’s how you can conduct research effectively:

  • Define the scope of your research : Determine what information is essential to the argument. This initial step will help you focus your research efforts and prevent irrelevant data.
  • Identify key sources : Begin with scholarly databases and academic journals that offer peer-reviewed articles. These sources provide credible, authoritative information that can be crucial for academic writing.
  • Use precise keywords : When searching for information, use specific keywords related to your essay topic. Precision here will help find the most relevant articles and studies, reducing time spent on unnecessary reading.
  • Evaluate sources : Assess the relevance and reliability of each source. Check the publication date to ensure the information is current and relevant to your topic.
  • Take notes efficiently : As you research, jot down important points, quotes, and references. Organize these notes according to the sections in your essay to make writing faster.
  • Synthesize information : Combine information from multiple sources to build a strong argument. This will allow you to write comprehensively and with fewer words, as each sentence carries more weight.

5. Improve Your Paragraph Structure

Streamlining paragraphs can make your essay shorter and more digestible for the reader. With a well-structured paragraph, you can focus on a single idea supported by concise statements.

Begin each paragraph with a topic sentence that clearly states the main idea. This sentence sets the direction and tone, letting the reader know what to expect. It also helps ensure that every following sentence relates directly to the main idea.

Condense supporting information by merging ideas that logically coexist within a single sentence or phrase. After that, evaluate each sentence for its contribution to the paragraph’s main idea. Remove any information that is repeated or goes into too much detail.

Focus on providing evidence and explanations that directly support the main point. You should also end each paragraph with a sentence that reinforces the main idea and potentially links to the next paragraph. This creates smooth transitions and keeps the essay focused and cohesive.

6. Refine the Introduction and Conclusion

These sections frame your essay and influence how your arguments are perceived. Here are some ways to keep them concise yet effective.

Introduction

The introduction should be engaging and concise, clearly stating the purpose and scope of your essay. Begin with a hook that grabs the reader’s attention, followed by background information that sets the context. Incorporate your thesis statement early on, ideally at the end of the intro.

The conclusion needs to reinforce the thesis. Summarize key points in the essay and show how they support the thesis. Provide a final thought that leaves the reader with something to ponder.

Also, remember to keep it tight – the conclusion isn’t a place for introducing new ideas. It should wrap up the ones you presented and prompt the reader to pose their own questions.

7. Edit and Proofread

Keep your essay concise and error-free by allocating ample time for editing and proofreading. These processes scrutinize your work at different levels, from the overall structure to word choices and punctuation. Here’s how you can go about it:

Start by reading through your entire paper to get a feel for its flow and coherence. Check if all paragraphs support your thesis statement and if section transitions are smooth. This will help you spot areas where the argument might be weak, or wording could be clearer.

Focus next on paragraph structure. Ensure each paragraph sticks to one main idea and that all sentences directly support the idea. Remove any repetitive or irrelevant sentences that don’t add value.

Then, look for clarity and style. Replace complex words with simpler alternatives to maintain readability. Keep your tone consistent throughout the paper. Adjust the sentence length and structure to enhance the flow and make it more engaging.

Proofreading

Proofreading comes after editing. The focus here is catching typing errors, grammatical mistakes, and inconsistent formatting. It’s always best to proofread with fresh eyes, so consider taking a break before this step.

Use tools like spell checkers, but don’t rely solely on them. Read your essay aloud or have someone else review it. Hearing the words can help you catch errors you may have missed.

Lastly, check for punctuation errors and ensure all citations and references are formatted according to the required academic style. This and all of the above are areas in which AI can help get the job done with speed and precision.

Why You Might Need to Shorten Your Essay

Ever heard the expression “less is more”? When it comes to academic writing, it normally is. Keeping your essays concise offers several benefits:

  • Enhances clarity : A shorter essay forces you to focus on the main points and critical arguments, reducing the risk of going off-topic. This clarity makes your writing more impactful and easier for the reader to follow.
  • Meets word limits : Many academic assignments have a maximum word count. Learning to express your thoughts concisely helps you stay within these limits without sacrificing essential content.
  • Saves time : For both the writer and the reader, shorter essays take less time to write, revise, and read. This efficiency is especially valuable in academic settings where time is usually limited.
  • Increases engagement : Readers are more likely to stay engaged with a document that gets to the point quickly. Lengthy texts can deter readers, especially if the content has unnecessary words or redundant points.
  • Improves writing skills : Shortening essays helps refine your writing skills. You become better at identifying and eliminating fluff, focusing instead on what really adds value to your paper.

Overall, adopting a more succinct writing style helps you meet academic requirements and polish your communication skills.

Why Use Smodin To Shorten an Essay

Using AI-powered platforms like Smodin to shorten your essay is both the simplest and the least time-consuming method available. Here’s why you should probably make Smodin your go-to essay shortener:

  • Efficiency : Smodin eases the editing process, using advanced algorithms to quickly identify areas where content can be condensed without losing meaning.
  • Accuracy : With its powerful AI, Smodin ensures that the essence of your essays stays intact while getting rid of unnecessary words, making your writing more precise.
  • Ease of use : Smodin is user-friendly, making it accessible even to those who aren’t the most tech-savvy. Its easy-to-grasp interface allows for seamless navigation and operation.

Smodin’s offerings

  • Rewriter : Available in over 50 languages, this tool helps rewrite text to be more concise.
  • Article Writer : Assists in drafting articles that are crisp and to the point.
  • Plagiarism and Auto Citation : Ensures your essay is original and correctly cited, which is crucial in academic writing.
  • Language Detection : Identifies the language of the text, ensuring the right adjustments are made for clarity.

All these tools and more are what make Smodin an excellent choice for academics looking to reduce the length of their essays.

Final Thoughts

Word counts can be a real headache, especially when you need to say a lot with a little. Thankfully, by identifying unnecessary words, tightening your sentences, and using tools like Smodin, you can make your essay concise without losing its meaning. Remember, a shorter essay doesn’t just meet word limits; and it’s clear, more compelling, and more likely to keep your reader engaged.

Keep it short, keep it sweet, and make every word count! Get started for free right now with Smodin.

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  • CAREER BRIEF
  • 08 May 2019

Toolkit: How to write a great paper

A clear format will ensure that your research paper is understood by your readers. Follow:

1. Context — your introduction

2. Content — your results

3. Conclusion — your discussion

Plan your paper carefully and decide where each point will sit within the framework before you begin writing.

how to make a research paper shorter

Collection: Careers toolkit

Straightforward writing

Scientific writing should always aim to be A, B and C: Accurate, Brief, and Clear. Never choose a long word when a short one will do. Use simple language to communicate your results. Always aim to distill your message down into the simplest sentence possible.

Choose a title

A carefully conceived title will communicate the single core message of your research paper. It should be D, E, F: Declarative, Engaging and Focused.

Conclusions

Add a sentence or two at the end of your concluding statement that sets out your plans for further research. What is next for you or others working in your field?

Find out more

See additional information .

doi: https://doi.org/10.1038/d41586-019-01362-9

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Research Method

Home » Research Paper – Structure, Examples and Writing Guide

Research Paper – Structure, Examples and Writing Guide

Table of Contents

Research Paper

Research Paper

Definition:

Research Paper is a written document that presents the author’s original research, analysis, and interpretation of a specific topic or issue.

It is typically based on Empirical Evidence, and may involve qualitative or quantitative research methods, or a combination of both. The purpose of a research paper is to contribute new knowledge or insights to a particular field of study, and to demonstrate the author’s understanding of the existing literature and theories related to the topic.

Structure of Research Paper

The structure of a research paper typically follows a standard format, consisting of several sections that convey specific information about the research study. The following is a detailed explanation of the structure of a research paper:

The title page contains the title of the paper, the name(s) of the author(s), and the affiliation(s) of the author(s). It also includes the date of submission and possibly, the name of the journal or conference where the paper is to be published.

The abstract is a brief summary of the research paper, typically ranging from 100 to 250 words. It should include the research question, the methods used, the key findings, and the implications of the results. The abstract should be written in a concise and clear manner to allow readers to quickly grasp the essence of the research.

Introduction

The introduction section of a research paper provides background information about the research problem, the research question, and the research objectives. It also outlines the significance of the research, the research gap that it aims to fill, and the approach taken to address the research question. Finally, the introduction section ends with a clear statement of the research hypothesis or research question.

Literature Review

The literature review section of a research paper provides an overview of the existing literature on the topic of study. It includes a critical analysis and synthesis of the literature, highlighting the key concepts, themes, and debates. The literature review should also demonstrate the research gap and how the current study seeks to address it.

The methods section of a research paper describes the research design, the sample selection, the data collection and analysis procedures, and the statistical methods used to analyze the data. This section should provide sufficient detail for other researchers to replicate the study.

The results section presents the findings of the research, using tables, graphs, and figures to illustrate the data. The findings should be presented in a clear and concise manner, with reference to the research question and hypothesis.

The discussion section of a research paper interprets the findings and discusses their implications for the research question, the literature review, and the field of study. It should also address the limitations of the study and suggest future research directions.

The conclusion section summarizes the main findings of the study, restates the research question and hypothesis, and provides a final reflection on the significance of the research.

The references section provides a list of all the sources cited in the paper, following a specific citation style such as APA, MLA or Chicago.

How to Write Research Paper

You can write Research Paper by the following guide:

  • Choose a Topic: The first step is to select a topic that interests you and is relevant to your field of study. Brainstorm ideas and narrow down to a research question that is specific and researchable.
  • Conduct a Literature Review: The literature review helps you identify the gap in the existing research and provides a basis for your research question. It also helps you to develop a theoretical framework and research hypothesis.
  • Develop a Thesis Statement : The thesis statement is the main argument of your research paper. It should be clear, concise and specific to your research question.
  • Plan your Research: Develop a research plan that outlines the methods, data sources, and data analysis procedures. This will help you to collect and analyze data effectively.
  • Collect and Analyze Data: Collect data using various methods such as surveys, interviews, observations, or experiments. Analyze data using statistical tools or other qualitative methods.
  • Organize your Paper : Organize your paper into sections such as Introduction, Literature Review, Methods, Results, Discussion, and Conclusion. Ensure that each section is coherent and follows a logical flow.
  • Write your Paper : Start by writing the introduction, followed by the literature review, methods, results, discussion, and conclusion. Ensure that your writing is clear, concise, and follows the required formatting and citation styles.
  • Edit and Proofread your Paper: Review your paper for grammar and spelling errors, and ensure that it is well-structured and easy to read. Ask someone else to review your paper to get feedback and suggestions for improvement.
  • Cite your Sources: Ensure that you properly cite all sources used in your research paper. This is essential for giving credit to the original authors and avoiding plagiarism.

Research Paper Example

Note : The below example research paper is for illustrative purposes only and is not an actual research paper. Actual research papers may have different structures, contents, and formats depending on the field of study, research question, data collection and analysis methods, and other factors. Students should always consult with their professors or supervisors for specific guidelines and expectations for their research papers.

Research Paper Example sample for Students:

Title: The Impact of Social Media on Mental Health among Young Adults

Abstract: This study aims to investigate the impact of social media use on the mental health of young adults. A literature review was conducted to examine the existing research on the topic. A survey was then administered to 200 university students to collect data on their social media use, mental health status, and perceived impact of social media on their mental health. The results showed that social media use is positively associated with depression, anxiety, and stress. The study also found that social comparison, cyberbullying, and FOMO (Fear of Missing Out) are significant predictors of mental health problems among young adults.

Introduction: Social media has become an integral part of modern life, particularly among young adults. While social media has many benefits, including increased communication and social connectivity, it has also been associated with negative outcomes, such as addiction, cyberbullying, and mental health problems. This study aims to investigate the impact of social media use on the mental health of young adults.

Literature Review: The literature review highlights the existing research on the impact of social media use on mental health. The review shows that social media use is associated with depression, anxiety, stress, and other mental health problems. The review also identifies the factors that contribute to the negative impact of social media, including social comparison, cyberbullying, and FOMO.

Methods : A survey was administered to 200 university students to collect data on their social media use, mental health status, and perceived impact of social media on their mental health. The survey included questions on social media use, mental health status (measured using the DASS-21), and perceived impact of social media on their mental health. Data were analyzed using descriptive statistics and regression analysis.

Results : The results showed that social media use is positively associated with depression, anxiety, and stress. The study also found that social comparison, cyberbullying, and FOMO are significant predictors of mental health problems among young adults.

Discussion : The study’s findings suggest that social media use has a negative impact on the mental health of young adults. The study highlights the need for interventions that address the factors contributing to the negative impact of social media, such as social comparison, cyberbullying, and FOMO.

Conclusion : In conclusion, social media use has a significant impact on the mental health of young adults. The study’s findings underscore the need for interventions that promote healthy social media use and address the negative outcomes associated with social media use. Future research can explore the effectiveness of interventions aimed at reducing the negative impact of social media on mental health. Additionally, longitudinal studies can investigate the long-term effects of social media use on mental health.

Limitations : The study has some limitations, including the use of self-report measures and a cross-sectional design. The use of self-report measures may result in biased responses, and a cross-sectional design limits the ability to establish causality.

Implications: The study’s findings have implications for mental health professionals, educators, and policymakers. Mental health professionals can use the findings to develop interventions that address the negative impact of social media use on mental health. Educators can incorporate social media literacy into their curriculum to promote healthy social media use among young adults. Policymakers can use the findings to develop policies that protect young adults from the negative outcomes associated with social media use.

References :

  • Twenge, J. M., & Campbell, W. K. (2019). Associations between screen time and lower psychological well-being among children and adolescents: Evidence from a population-based study. Preventive medicine reports, 15, 100918.
  • Primack, B. A., Shensa, A., Escobar-Viera, C. G., Barrett, E. L., Sidani, J. E., Colditz, J. B., … & James, A. E. (2017). Use of multiple social media platforms and symptoms of depression and anxiety: A nationally-representative study among US young adults. Computers in Human Behavior, 69, 1-9.
  • Van der Meer, T. G., & Verhoeven, J. W. (2017). Social media and its impact on academic performance of students. Journal of Information Technology Education: Research, 16, 383-398.

Appendix : The survey used in this study is provided below.

Social Media and Mental Health Survey

  • How often do you use social media per day?
  • Less than 30 minutes
  • 30 minutes to 1 hour
  • 1 to 2 hours
  • 2 to 4 hours
  • More than 4 hours
  • Which social media platforms do you use?
  • Others (Please specify)
  • How often do you experience the following on social media?
  • Social comparison (comparing yourself to others)
  • Cyberbullying
  • Fear of Missing Out (FOMO)
  • Have you ever experienced any of the following mental health problems in the past month?
  • Do you think social media use has a positive or negative impact on your mental health?
  • Very positive
  • Somewhat positive
  • Somewhat negative
  • Very negative
  • In your opinion, which factors contribute to the negative impact of social media on mental health?
  • Social comparison
  • In your opinion, what interventions could be effective in reducing the negative impact of social media on mental health?
  • Education on healthy social media use
  • Counseling for mental health problems caused by social media
  • Social media detox programs
  • Regulation of social media use

Thank you for your participation!

Applications of Research Paper

Research papers have several applications in various fields, including:

  • Advancing knowledge: Research papers contribute to the advancement of knowledge by generating new insights, theories, and findings that can inform future research and practice. They help to answer important questions, clarify existing knowledge, and identify areas that require further investigation.
  • Informing policy: Research papers can inform policy decisions by providing evidence-based recommendations for policymakers. They can help to identify gaps in current policies, evaluate the effectiveness of interventions, and inform the development of new policies and regulations.
  • Improving practice: Research papers can improve practice by providing evidence-based guidance for professionals in various fields, including medicine, education, business, and psychology. They can inform the development of best practices, guidelines, and standards of care that can improve outcomes for individuals and organizations.
  • Educating students : Research papers are often used as teaching tools in universities and colleges to educate students about research methods, data analysis, and academic writing. They help students to develop critical thinking skills, research skills, and communication skills that are essential for success in many careers.
  • Fostering collaboration: Research papers can foster collaboration among researchers, practitioners, and policymakers by providing a platform for sharing knowledge and ideas. They can facilitate interdisciplinary collaborations and partnerships that can lead to innovative solutions to complex problems.

When to Write Research Paper

Research papers are typically written when a person has completed a research project or when they have conducted a study and have obtained data or findings that they want to share with the academic or professional community. Research papers are usually written in academic settings, such as universities, but they can also be written in professional settings, such as research organizations, government agencies, or private companies.

Here are some common situations where a person might need to write a research paper:

  • For academic purposes: Students in universities and colleges are often required to write research papers as part of their coursework, particularly in the social sciences, natural sciences, and humanities. Writing research papers helps students to develop research skills, critical thinking skills, and academic writing skills.
  • For publication: Researchers often write research papers to publish their findings in academic journals or to present their work at academic conferences. Publishing research papers is an important way to disseminate research findings to the academic community and to establish oneself as an expert in a particular field.
  • To inform policy or practice : Researchers may write research papers to inform policy decisions or to improve practice in various fields. Research findings can be used to inform the development of policies, guidelines, and best practices that can improve outcomes for individuals and organizations.
  • To share new insights or ideas: Researchers may write research papers to share new insights or ideas with the academic or professional community. They may present new theories, propose new research methods, or challenge existing paradigms in their field.

Purpose of Research Paper

The purpose of a research paper is to present the results of a study or investigation in a clear, concise, and structured manner. Research papers are written to communicate new knowledge, ideas, or findings to a specific audience, such as researchers, scholars, practitioners, or policymakers. The primary purposes of a research paper are:

  • To contribute to the body of knowledge : Research papers aim to add new knowledge or insights to a particular field or discipline. They do this by reporting the results of empirical studies, reviewing and synthesizing existing literature, proposing new theories, or providing new perspectives on a topic.
  • To inform or persuade: Research papers are written to inform or persuade the reader about a particular issue, topic, or phenomenon. They present evidence and arguments to support their claims and seek to persuade the reader of the validity of their findings or recommendations.
  • To advance the field: Research papers seek to advance the field or discipline by identifying gaps in knowledge, proposing new research questions or approaches, or challenging existing assumptions or paradigms. They aim to contribute to ongoing debates and discussions within a field and to stimulate further research and inquiry.
  • To demonstrate research skills: Research papers demonstrate the author’s research skills, including their ability to design and conduct a study, collect and analyze data, and interpret and communicate findings. They also demonstrate the author’s ability to critically evaluate existing literature, synthesize information from multiple sources, and write in a clear and structured manner.

Characteristics of Research Paper

Research papers have several characteristics that distinguish them from other forms of academic or professional writing. Here are some common characteristics of research papers:

  • Evidence-based: Research papers are based on empirical evidence, which is collected through rigorous research methods such as experiments, surveys, observations, or interviews. They rely on objective data and facts to support their claims and conclusions.
  • Structured and organized: Research papers have a clear and logical structure, with sections such as introduction, literature review, methods, results, discussion, and conclusion. They are organized in a way that helps the reader to follow the argument and understand the findings.
  • Formal and objective: Research papers are written in a formal and objective tone, with an emphasis on clarity, precision, and accuracy. They avoid subjective language or personal opinions and instead rely on objective data and analysis to support their arguments.
  • Citations and references: Research papers include citations and references to acknowledge the sources of information and ideas used in the paper. They use a specific citation style, such as APA, MLA, or Chicago, to ensure consistency and accuracy.
  • Peer-reviewed: Research papers are often peer-reviewed, which means they are evaluated by other experts in the field before they are published. Peer-review ensures that the research is of high quality, meets ethical standards, and contributes to the advancement of knowledge in the field.
  • Objective and unbiased: Research papers strive to be objective and unbiased in their presentation of the findings. They avoid personal biases or preconceptions and instead rely on the data and analysis to draw conclusions.

Advantages of Research Paper

Research papers have many advantages, both for the individual researcher and for the broader academic and professional community. Here are some advantages of research papers:

  • Contribution to knowledge: Research papers contribute to the body of knowledge in a particular field or discipline. They add new information, insights, and perspectives to existing literature and help advance the understanding of a particular phenomenon or issue.
  • Opportunity for intellectual growth: Research papers provide an opportunity for intellectual growth for the researcher. They require critical thinking, problem-solving, and creativity, which can help develop the researcher’s skills and knowledge.
  • Career advancement: Research papers can help advance the researcher’s career by demonstrating their expertise and contributions to the field. They can also lead to new research opportunities, collaborations, and funding.
  • Academic recognition: Research papers can lead to academic recognition in the form of awards, grants, or invitations to speak at conferences or events. They can also contribute to the researcher’s reputation and standing in the field.
  • Impact on policy and practice: Research papers can have a significant impact on policy and practice. They can inform policy decisions, guide practice, and lead to changes in laws, regulations, or procedures.
  • Advancement of society: Research papers can contribute to the advancement of society by addressing important issues, identifying solutions to problems, and promoting social justice and equality.

Limitations of Research Paper

Research papers also have some limitations that should be considered when interpreting their findings or implications. Here are some common limitations of research papers:

  • Limited generalizability: Research findings may not be generalizable to other populations, settings, or contexts. Studies often use specific samples or conditions that may not reflect the broader population or real-world situations.
  • Potential for bias : Research papers may be biased due to factors such as sample selection, measurement errors, or researcher biases. It is important to evaluate the quality of the research design and methods used to ensure that the findings are valid and reliable.
  • Ethical concerns: Research papers may raise ethical concerns, such as the use of vulnerable populations or invasive procedures. Researchers must adhere to ethical guidelines and obtain informed consent from participants to ensure that the research is conducted in a responsible and respectful manner.
  • Limitations of methodology: Research papers may be limited by the methodology used to collect and analyze data. For example, certain research methods may not capture the complexity or nuance of a particular phenomenon, or may not be appropriate for certain research questions.
  • Publication bias: Research papers may be subject to publication bias, where positive or significant findings are more likely to be published than negative or non-significant findings. This can skew the overall findings of a particular area of research.
  • Time and resource constraints: Research papers may be limited by time and resource constraints, which can affect the quality and scope of the research. Researchers may not have access to certain data or resources, or may be unable to conduct long-term studies due to practical limitations.

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how to make a research paper shorter

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We have lots more on the site to show you. You've only seen one page. Check out this post which is one of the most popular of all time.

How to Add Length to a Research Paper: Tips and Tricks

When writing research papers most students struggle with two things the most: making their papers shorter and making them longer. We will focus on the latter, because if your paper isn’t long enough you will not have enough content to make good edits, or even make anything shorter. When you’re starting out, the struggle is often with adding length.Then as you mature as a student the more difficult challenge becomes removing content from papers and editing for clarity. Don’t get discouraged if you are facing the need to add length, it takes a long time to get to the point of automatically writing too much. Dave talks in his vlog below about his own experiences adding length to research papers when when he was first started out:

This post was written by Stephanie A. Bosco-Ruggiero (PhD candidate in Social Work at Fordham University Graduate School of Social Service) and Jessica Russell ( freelance writer) on behalf of Dave Maslach for the R3ciprocity project (Check out the YouTube Channel or the writing feedback software ). R3ciprocity helps students, faculty, and research folk by providing a real and authentic look into doing research. It provides solutions and hope to researchers around the world.

There are many different ways of writing a research paper and your professor will have his/her own rubric for what must be included and how the paper will be graded. Graduate level research papers may tend to be more like lengthy literature reviews where you review and synthesize what is already known about your topic. At the doctoral level (or graduate level), you may be proposing an actual research study. Still other papers have their own requirements and rubrics. ( If you like this post, you might want to read this one on how professors grade papers. )

There are many places where you can add content to different types of papers. It is actually more difficult to make a research paper shorter, but there are simple tricks to make your research paper longer. This post discusses some tips and tricks for adding length to doctoral level research papers, in particular, but it also will help you find ways to add length to other types of papers as well, including at the graduate level.  

Choosing a good topic may be a process, not the first step

It is important that you’ve chosen a good research topic. By making sure to do many of the following things we describe when writing, you’ll find out if you have chosen well. Sometimes taking these steps will cause you to change your path, and sometimes (hopefully more often) it will truly help you not only add length to your paper, but get into the really important aspects of what you want and need to say. 

What should I write in the introduction ?

Most research papers begin with an introduction, but this section should only be two to four paragraphs. You really don’t want to go on and on in the beginning of the paper because it really is for summarizing your topic, what the aim of your study is, and perhaps why it is important to look at this issue or research question. You can say more in the background and/or literature review about the context, state of the art in practice, or what knowledge there is to date on this topic. Here are some tips for writing more about the context of your study in one of the earlier sections of your paper (e.g. background, aims and scope literature review, etc.)

What is the context of your research?

Write about the who, what, when, where, how, and why of your problem, issue, or phenomenon. Write 2-3 sentences for each W or the how. Who does it affect, who can affect the outcomes, who needs to be involved? What is the objective or the strategy of your paper? What has happened before, what is happening now, and what could be happening in the future? Where is this problem or issue occurring, and where are its effects happening most? Where is this topic needed and who needs to be discussing it? And why is that? Why is it happening, why should we care, and why has this topic not been at the forefront? These are just some of the questions you can ask yourself to flesh out the context  of your research questions and research paper. 

Why is your research or topic important ?

Write about why this issue or problem is important and what the potential implications are of your study. After writing a few sentences about who, what, where, and why, you will start to see where you can expand upon what you’ve already started to discuss about context. Why is this study or research topic important, who does it affect, where are the implications most evident, and what needs to happen in order to affect change? Then switch those questions around to consider more views on the topic. Dig deep into every avenue of thought and hypothesis on this topic. This alone will give you something to write about.  (Here is a great blog post on choosing research topics that you should read) .

What are your research questions and hypotheses?

This the place where brevity is best. You do not want to provide detail to back up your hypotheses in this section. Your hypotheses emerge from your review of the literature and your knowledge of the field. Your research questions are expanded on in your background and literature review. You have taken a journey toward developing specific research questions and hypotheses if you are proposing a study. Your research questions and hypotheses may be refined several times, but that is ok. Just make sure they are clear and to the point. 

What is your literature review telling you about what is known and what you might find (if you are doing a study)?

The background and literature sections of your paper are great places for discussing the context of the study or phenomenon and for expanding on what is known in the field about this topic, and /or what the different hypotheses or trains of thought are about how it works or why it is relevant. Your literature review should not be a simple list (like an annotated bibliography) of studies that have preceded yours and what the findings were. Rather it should synthesize similar findings, discuss emerging themes, and show how knowledge to date about the subject suggests your hypothesis might be true. If you need help with literature reviews, you might want to read this blog post:

What Is A Literature Review (In A PhD Or Graduate School)? A How to Guide

You might also want to discuss similar theories about the phenomenon, as well as what is not yet known and needs to be studied. There is a lot to write about when looking at the literature and history of your topic, but be careful not to list every study or paper known to man about the topic. Look back about ten years and highlight the most important studies or information. As you mature as a student, this is the section where you tend to write too much and can edit down.

Write a detailed description of 1-2 pages about how things are thought to work. Focus on what is known. When we work from what we know, we can see the gaps that need to be addressed. Do more research if you are struggling on the history and current events relevant to your topic. Search different sources to learn up to the date details on current happenings in your field of study. As you are covering this, more questions and thoughts about the topic will pop into your head. Make sure to take notes and jot down ideas, even if they are small. You never know which thought will lead to a few pages of content. Make sure you establish why your research question and study are necessary and important.

Which theories provide the foundation for your research questions, hypotheses or study?

Your paper may require you to propose or critique a theory. Write where, what, and why the theory is important. Aim for writing 2-3 sentences for each W. Your theory, or someone else’s theory, is the basis of why you’re studying this topic. Start off with a few sentences and then expand upon each sentence. As you are writing, you will develop ideas you may not have thought about initially. It is important to really get into the meat of your theory. Your paper is meant to prove your theory, or it will disprove it as you’re working and cause you to take new avenues and thought patterns to figure out what needs to change. 

Note why you have not chosen a different theory to focus on. It helps to talk about why your theory is important. Why are you writing this, why do you believe in it and why others should read your paper. What is it about this theory that made it something you chose to write about, chose to study and research? And why should others take notice and consider everything you’re saying? Why should it lead to others choosing this to research and study? What led you down this path, what were some of the topics that you didn’t choose, but steered your research towards this theory? 

Where can you go deeper with your analysis ?

Look for multiple levels of analysis. What happens when you zoom in and zoom out of what you are looking at? A great example Dave talks about is looking at your hand. It’s a hand, you know it has bones and muscles and tendons, so he says, “ I can look at my hand and I can see it as a hand but I can think about it in terms of the molecular structure that’s behind it. I can think of it in terms of cells that are behind it. You know, I can think of it in terms of the different wrinkles and wrinkle patterns; I have lots of fingerprint patterns. ” The multiple levels of analysis he is describing will give you a rich, multifaceted, understanding and written description of your topic. Writing a paragraph or two about each level of analysis of your topic and/or a theory will really tease out the details and add length and clarity to your paper. 

Where can you define for clarity and understanding ?

Write definitions for each of the ideas you introduce in your paper. Each definition will add one paragraph to the paper. Not everyone who reads your paper is an expert on your theory or topic. It is often helpful to add definitions both to aid understanding and for adding length. Just make sure not to add superfluous definitions to add length where no additional understanding is really needed. Ask yourself if you weren’t in this field, talking about this topic regularly, what would you need to understand better in order to understand the whole? Define those topics and ideas, expand upon those topics and ideas with the intention of making them more clear to the reader and as a basis for topical understanding. 

Have you described your research methods thoroughly ?

Why is the method that you are using important and the best method for addressing your research question? How is it different from other methods? What are the key assumptions in your research method? Why can’t you use another method? It’s important to keep notes about your methods. These notes will help you describe your methods, conduct your research and discuss the importance of your methods. You can talk about how other methods vary as well as how other methods failed to yield satisfying answers. Explain how different methods led you to your current method and why the changes were necessary. Spend time discussing how you came to where you are now in your research and what brought you to this place. Taking time to expand on these thoughts in your paper to add length and detail, and to document the evolution in your thinking about how to approach this study. 

What do you think? Write it down 

Not everything has to be cited. If you have a clever idea that is novel, about 75% of your paper will have citations but 25% of your paper will be original . Record your sources and make good citations. But make sure to expand upon your thought patterns on the topic. Yes, you have chosen a research topic and are required to write about it, but you are here for a reason. You have chosen your topic, perhaps created a theory or hypothesis, and are at the point of writing about all you’ve learned. Don’t just talk about what others say, think, and have done. Talk about all the ideas you’ve thought about (take notes!) along the way. 

Research sparks ideas and creativity. This provides avenues for new thoughts and theories. Research sparks more research. Learning and discovering new ways to do things, change things, save lives, increase productivity, provide meaning, and spark more in the minds and lives of others are half of the reasons you do what you do. Talk about what you think about everything you’ve learned and where you think there are more avenues for further study. 

Dave says that you know your own ideas. He says it’s important to look for ways that you can add things that are uniquely your own insights because not everything has to be cited in a research paper. The general rule is you want 60-80% of your content to be based on others’ ideas and work. The remaining 20-40% is your own thinking, theorizing, and synthesizing. You can go on a tangent sometimes about different things, and talk about those different things that you find interesting. It will add to the particular context so if you do that you’re going to have a much more original paper and it’s going to seem a little more clever because nobody else is talking about these ideas. 

Discussion: Have you described several mechanisms that might explain what you are looking at ?

There might be several reasons for the change you have observed or are trying to explain. You are seeing change or certain information in your work. Take time to describe those mechanisms, but also look at different mechanisms that might help you and others understand what you are seeing. Looking at it from different viewpoints or through another lens will give you a better understanding of the who, what, where and why of your topic. Considering other mechanisms can help you as you do the research because then you are really thinking about what is happening and why. And, what you may not have considered before or dismissed off hand will become important, not only to your theory, but to your research in general. 

There are many places where you can add length to a paper. At the doctoral level especially, great detail is expected. Just do not write your paper as if you are trying to think of things to say or write about. It will be obvious. You have enough to talk about, even if you don’t realize it at first, to have a paper with sufficient depth and breadth.

If you enjoyed this post, check out these other posts at  http://r3ciprocity.com

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Home / Guides / Writing Guides / Writing Tips / How to Make an Essay Longer the Smart Way

How to Make an Essay Longer the Smart Way

Meeting an essay’s required page or word count can sometimes be a struggle, especially if you’re juggling multiple papers or exams. In a pinch, students often rely on tricks like increasing margin size or making their font slightly bigger. Though these tricks do increase page length, there are easier (and smarter) ways to write a longer, high-quality essay. Making a paper meet minimum word or page counts doesn’t have to be an agonizing process—you can add length while also adding clarity and depth.

Here are 10 tips on how you can write a longer and a smarter essay, even if the deadline is fast approaching:

Tip #1: Look Back at Your Prompt/Rubric/etc.

If you’ve been provided a comprehensive prompt or rubric for an essay, read it, and read it again. Think about the following:

  • Did you answer all of the questions in the prompt?
  • Did you provide supporting evidence to back up whatever claims you made?
  • Did you leave out any information that might increase the reader’s understanding of your argument?
  • Did you meet all requirements (besides length) for the paper?

If the answer isn’t a decisive “yes” to every question on this list, go back and revise.

Tip #2: Go Back Through Your Introduction and Conclusion

Often times, ideas evolve while writing a paper. If the first thing you wrote was the introduction, go back and reread the first paragraph. You might decide that you left out key information that aids the reader in understanding your argument. When looking back on the conclusion, make sure you’ve both summarized the main points within the essay and provided your reader with a solution to consider. If you don’t feel you’ve done this, go back through and revise the paper.

Tip #3: Have Someone Proofread Your Essay

Even if you’re short on time ask a friend, sibling, or parent to read through your paper, specifically noting any points they find confusing. Then, go back and revise the parts that were unclear, adding in more information to provide readers with further clarity. You have a more comprehensive understanding of what you’re writing about than your reader, so having someone else look over your paper can be a helpful way to ensure that you haven’t missed any important details.

Tip #4: Use Quotations

Chances are, you have already used quotes in your paper. Quotations are a great way to enhance your argument while also driving up a paper’s word count, but don’t add quotes just for the sake of doing so. If you’re short on words, read through your source materials again to see if you’ve missed any valuable quotes. You can also do a little more research to see if there are any other sources you can add to provide the reader with more evidence toward your argument. Longer quotes aren’t necessarily better, but if you’re really in a bind, you might want to lengthen some of the quotes that are already included.

Tip #5: Review Your Outline

Did you make an outline to plan the essay when you first started? Go back through that initial outline and make sure you’ve hit all of your intended points. It’s possible that you’ve left out an important piece of your argument that would both increase page count and make for a better essay.

Tip #6: Include More Transitional Phrases

Graders often look for traditional words linking sentences to each other, like “therefore,” “even though”, and “on the other hand.” Read through your essay and make sure the sentences flow smoothly into each other. If they don’t, go back and add in transitional phrases like the ones listed above. Your writing will be easier to read, and you’ll get closer to the minimum page requirement in the process.

Tip #7: Read Your Paper Out Loud

This might sound like a silly tip, but when you read your paper out loud, you become increasingly aware of any grammatical or syntactical issues. When you rephrase sentences to fix these, you might end up increasing the paper length a bit. In the process of reading out loud, you also might realize that you didn’t include sufficient details within a particular paragraph. If that’s the case, go back in and add more to increase length.

Tip #8: Take a Break From Your Essay

You’ve probably been staring at your computer screen for hours, hoping words will magically pop into your head. Take a break. Eat a snack, go for a walk, or talk to a friend on the phone. You’ll come back to the essay with a fresh perspective after some time away, and you might have new ideas after you’ve had time away from your paper.

Tip #9: Ask Your Instructor for Help

Most teachers, teaching assistants, and professors are willing to look over papers for students before the final submission date. If there is still time, ask if you can make an appointment to go over your paper or head over to office hours. Your instructor might offer tips on how to better answer the prompt, and this in turn may also increase the word count of the paper.

Tip #10: Use multiple examples to back up your argument

If you’ve only used one source or anecdote to explain a given point, find a second source to provide additional evidence for the reader. This method will help drive up a paper’s word count while also providing further support for your argument.

Although hitting a minimum page count can sometimes be challenging, you can do it the smart way by increasing the information you provide to the reader—there’s no reason to resort to tricks like increasing line spacing or font size. If you’re really in a bind at the last minute, you might want to break up some of your paragraphs. This increases length while also making text more manageable for a reader. But after going through the tips on this list, your paper should be adequate in length without you having to even consider spacing.

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Jerz's Literacy Weblog (est. 1999)

Short research papers: how to write academic essays.

Jerz > Writing > Academic > Research Papers [ Title | Thesis  | Blueprint  | Quoting | Citing |  MLA Format  ]

This document focuses on the kind of  short, narrowly-focused research papers that might be the final project in a freshman writing class or 200-level literature survey course.

In high school, you probably wrote a lot of personal essays (where your goal was to demonstrate you were engaged) and a lot of info-dump paragraphs (where your goal was to demonstrate you could remember and organize information your teacher told you to learn).

How is a college research essay different from the writing you did in high school?

This short video covers the same topic in a different way; I think the video and handout work together fairly well.

The assignment description your professor has already given you is your best source for understanding your specific writing task, but in general, a college research paper asks you to use evidence to defend some non-obvious, nuanced point about a complex topic.

Some professors may simply want you to explain a situation or describe a process; however, a more challenging task asks you to take a stand, demonstrating you can use credible sources to defend your original ideas.

Short Research Papers: How to Write Academic Essays

  • Choose a Narrow Topic
  • Use Sources Appropriately

Avoid Distractions

Outside the classroom, if I want to “research” which phone I should buy, I would start with Google.

I would watch some YouTube unboxing videos, and I might ask my friends on social media. I’d assume somebody already has written about or knows about the latest phones, and the goal of my “research” is to find what the people I trust think is the correct answer.

An entomologist might do “research” by going into the forest, and catching and observing hundreds or thousands of butterflies. If she had begun and ended her research by Googling for “butterflies of Pennsylvania” she would never have seen, with her own eyes, that unusual specimen that leads her to conclude she has discovered a new species.

Her goal as a field researcher is not to find the “correct answer” that someone else has already published. Instead, her goal is to add something new to the store of human knowledge — something that hasn’t been written down yet.

As an undergraduate with a few short months or weeks to write a research paper, you won’t be expected to discover a new species of butterfly, or convince everyone on the planet to accept what 99.9% of scientists say about vaccines or climate change, or to adopt your personal views on abortion, vaping, or tattoos.

But your professor will probably want you to read essays published by credentialed experts who are presenting their results to other experts, often in excruciating detail that most of us non-experts will probably find boring.

Your instructor probably won’t give the results of a random Google search the same weight as peer-reviewed scholarly articles from academic journals. (See “ Academic Journals: What Are They? “)

The best databases are not free, but your student ID will get you access to your school’s collection of databases, so you should never have to pay to access any source. (Your friendly school librarian will help you find out exactly how to access the databases at your school.)

1. Plan to Revise

Even a very short paper is the result of a process.

  • You start with one idea, you test it, and you hit on something better.
  • You might end up somewhere unexpected. If so, that’s good — it means you learned something.
  • If you’re only just starting your paper, and it’s due tomorrow, you have already robbed yourself of your most valuable resource — time.

Showcase your best insights at the beginning of your paper (rather than saving them for the end).

You won’t know what your best ideas are until you’ve written a full draft. Part of revision involves identifying strong ideas and making them more prominent, identifying filler and other weak material, and pruning it away to leave more room to develop your best ideas.

  • It’s normal, in a your very first “discovery draft,” to hit on a really good idea about two-thirds of the way through your paper.
  • But a polished academic paper is not a mystery novel. (A busy reader will not have the patience to hunt for clues.)
  • A thesis statement that includes a clear reasoning blueprint (see “ Blueprinting: Planning Your Essay “) will help your reader identify and follow your ideas.

Before you submit your draft, make sure  the title, the introduction, and the conclusion match . (I am amazed at how many students overlook this simple step.)

2. Choose a Narrow Topic

A short undergraduate research paper is not the proper occasion for you to tackle huge issues, such as, “Was  Hamlet Shakespeare’s Best Tragedy?” or “Women’s Struggle for Equality” or “How to Eliminate Racism.”  You won’t be graded down simply because you don’t have all the answers right away.  The trick is to  zoom in on one tiny little part of the argument .

Short Research Paper: Sample Topics

How would you improve each of these paper topics? (My responses are at the bottom of the page.)

  • Environmentalism in America
  • Immigration Trends in Wisconsin’s Chippewa Valley
  • Drinking and Driving
  • Local TV News
  • 10 Ways that Advertisers Lie to the Public
  • Athletes on College Campuses

3. Use Sources Appropriately

Unless you were asked to write an opinion paper or a reflection statement, your professor probably expects you to draw a topic from the assigned readings (if any).

  • Some students frequently get this  backwards — they write the paper first, then “look for quotes” from sources that agree with the opinions they’ve already committed to. (That’s not really doing research to learn anything new — that’s just looking for confirmation of what you already believe.)
  • Start with the readings, but don’t pad your paper with  summary .
  • Many students try doing most of their research using Google. Depending on your topic, the Internet may simply not have good sources available.
  • Go ahead and surf as you try to narrow your topic, but remember: you still need to cite whatever you find. (See: “ Researching Academic Papers .”)

When learning about the place of women in Victorian society, Sally is shocked to discover women couldn’t vote or own property.  She begins her paper by listing these and other restrictions, and adds personal commentary such as:

Women can be just as strong and capable as men are.  Why do men think they have the right to make all the laws and keep all the money, when women stay in the kitchen?  People should be judged by what they contribute to society, not by the kind of chromosomes they carry.

After reaching the required number of pages, she tacks on a conclusion about how women are still fighting for their rights today, and submits her paper.

  • during the Victorian period, female authors were being published and read like never before
  • the public praised Queen Victoria (a woman!) for making England a world empire
  • some women actually fought against the new feminists because they distrusted their motives
  • many wealthy women in England were downright nasty to their poorer sisters, especially the Irish.
  • Sally’s paper focused mainly on her general impression that sexism is unfair (something that she already believed before she started taking the course), but Sally has not engaged with the controversies or surprising details (such as, for instance, the fact that for the first time male writers were writing with female readers in mind; or that upperclass women contributed to the degradation of lower-class women).

On the advice of her professor, Sally revises her paper as follows:

Sally’s focused revision (right) makes  specific reference to a particular source , and uses a quote to introduce a point.  Sally still injects her own opinion, but she is offering specific comments on complex issues, not bumper-sticker slogans and sweeping generalizations, such as those given on the left.

Documenting Evidence

Back up your claims by  quoting reputable sources .  If you write”Recent research shows that…” or “Many scholars believe that…”, you are making a claim. You will have to back it up with authoritative evidence.  This means that the body of your paper must include references to the specific page numbers where you got your outside information. (If your document is an online source that does not provide page numbers, ask your instructor what you should do. There might be a section title or paragraph number that you could cite, or you might print out the article and count the pages in your printout.)

Avoid using words like “always” or “never,” since all it takes is a single example to the contrary to disprove your claim.  Likewise, be careful with words of causation and proof.  For example, consider the claim that television causes violence in kids.  The evidence might be that kids who commit crimes typically watch more television than kids who don’t.  But… maybe the reason kids watch more television is that they’ve dropped out of school, and are unsupervised at home. An unsupervised kid might watch more television, and also commit more crimes — but that doesn’t mean that the television is the cause of those crimes.

You don’t need to cite common facts or observations, such as “a circle has 360 degrees” or “8-tracks and vinyl records are out of date,” but you would need to cite claims such as “circles have religious and philosophical significance in many cultures” or “the sales of 8-track tapes never approached those of vinyl records.”

Don’t waste words referring directly to “quotes” and “sources.”

If you use words like “in the book  My Big Boring Academic Study , by Professor H. Pompous Windbag III, it says” or “the following quote by a government study shows that…” you are wasting words that would be better spent developing your ideas.

In the book  Gramophone, Film, Typewriter , by Fredrich A. Kittler, it talks about writing and gender, and says on page 186, “an omnipresent metaphor equated women with the white sheet of nature or virginity onto which a very male stylus could inscribe the glory of its authorship.”  As you can see from this quote, all this would change when women started working as professional typists.

The “it talks about” and “As you can see from this quote” are weak attempts to engage with the ideas presented by Kittler.  “In the book… it talks” is wordy and nonsensical (books don’t talk).

MLA style encourages you to  expend fewer words introducing your sources , and more words developing your own ideas.  MLA style involves just the author’s last name, a space ( not a comma), and then the page number.  Leave the author’s full name and the the title of the source for the Works Cited list at the end of your paper. Using about the same space as the original, see how MLA style helps an author devote more words to developing the idea more fully:

Before the invention of the typewriter, “an omnipresent metaphor” among professional writers concerned “a very male stylus” writing upon the passive, feminized “white sheet of nature or virginity” (Kittler 186).  By contrast, the word “typewriter” referred to the machine as well as the female typist who used it (183).

See “ Quotations: Integrating them in MLA-Style Papers. ”

Stay On Topic

It’s fairly normal to sit and stare at the computer screen for a while until you come up with a title, then pick your way through your topic, offering an extremely broad introduction (see  glittering generalities , below)..

  • You might also type in a few long quotations that you like.
  • After writing generalities and just poking and prodding for page or two,  you will eventually hit on a fairly good idea .
  • You will pursue it for a paragraph or two, perhaps throwing in another quotation.
  • By then, you’ll realize that you’ve got almost three pages written, so you will tack on a hasty conclusion.

Hooray, you’ve finished your paper! Well, not quite…

  • At the very least, you ought to  rewrite your title and introduction to match your conclusion , so it looks like the place you ended up was where you were intending to go all along.  You probably won’t get an A, because you’re still submitting two pages of fluff; but you will get credit for recognizing whatever you actually did accomplish.
  • To get an A, you should delete all that fluff,  use the “good idea” that you stumbled across as your new starting point , and keep going.   Even “good writers” have to work — beefing up their best ideas and shaving away the rest, in order to build a whole paper that serves the good idea, rather than tacking the good idea on at the end and calling it a day.

See:  Sally Slacker Writes a Paper , and  Sally’s Professor Responds

Avoid Glittering Generalities

Key: Research Paper Topics

15 thoughts on “ Short Research Papers: How to Write Academic Essays ”

Hi, I was searching for some information on how to write quality academic paper when I came across your awesome article on Short Research Papers: How to Writer Academic Essays ( https://jerz.setonhill.edu/writing/academic1/short-research-papers/ ) Great stuff!!! I especially like the way you recommend sticking to the 4 basics of writing academic essays. Very few students have mastered how to avoid distractions and focus on a single topic. Many students think that the broad, sweeping statements could give them better grades but they are wrong.

However, I came across a few links that didn’t seem to be working for you. Want me to forward you the short list I jotted down? Cheers Elias

I see some broken links in the comments, but otherwise I’m not sure what you mean.

I found the part about not using my personal opinion or generalities to be very helpful. I am currently writing a 2 page paper and was having a hard time keeping it short. Now I know why. Thanks. Stick to the facts.

This seem to be old but very relevant. Most of what you have stated are things my professor has stated during class trying to prepare us to write a short thesis reading this information verses hearing it was very helpful. You have done an awesome job! I just hope I can take this and apply it to my papers!

Great Post! Thank u!

Thank you for all your effort and help. You´ve taught me a number of things, especially on what college professors´ look for in assigning students short research papers. I am bookmarking your page, and using it as a reference.

Thank you kindly. YOU´VE HELPED A LOST STUDENT FIND HER WAY!

I appreaciate all the help your web site has given to me. I have referred to it many times. I think there may be a typo under the headline of AVOID GLITTERING GENERALITIES: “Throughout the ages, mankind has found many uses for salt. Ancient tribes used it preserve meat;” This is in no way a slight – I thought you might want to know. Please forgive me if I am incorrect. Thank you again – you rock!

You are right — I’ll fix it the next time I’m at my desktop. Thank you!

i would like to say thank you for your detailed information even though it takes time to read as well as we’ve got learnings out from it . even though it’s holiday next week our teacher assigned us to make a short research paper in accordance of our selected topic ! I’m hoping that we can make it cause if we can’t make it, right away, for sure we will get a grade’s that can drop our jaws ! :) ♥ tnx ! keep it up ! ♪♪

Sorry I have not done this for years

Hello I am the mother of a high school student that needs help doing a paper proposal for her senior project. Her topic is Photography. To be honest I have done this for years and I am trying to help, but i am completely lost. What can you recommend since she told me a little late and the paper is due tomorrow 11/11/11.

This page is designed for college students, but I am sure your daughter’s teacher has assigned readings that will guide your daughter through her homework.

Any paper that your daughter writes herself, even if it is late, will be a valuable learning experience — showing her the value of managing her time better for the next time, and preparing her for the day when she will have to tackle grown-up problems on her own.

I am having a hard time with my government essay. I am 55 taking a college course for the first time, and I barely passed high school. Last year I took this course wrote the essay, and did many things wrong. It was all in the typing. I had good story line, excellent site words, and good points of arguments. It wasn’t right on paper. My format is off. Where can I find and print a format. also I need to learn site words.

Most teachers will provide a model to follow. If it’s not already part of the assignment instructions, you could ask your prof. Better yet, bring a near-complete draft to your prof’s office hours, a few days before the due date, and ask for feedback. Your school probably has a writing center or tutoring center, too.

I would like to thank you for such detailed information. I am not a native speaker and I am doing a research paper;so, as you may think, it is really a hard job for me. A friend of mine who saw my draft of Lit. Rev asked me what type of citation format i was using, MLA or APA and I was puzzeled; then I decided to check the net and came across to this! It is being such a help Elsa

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  • A Research Guide
  • Research Paper Guide

How to Make a Research Paper Longer?

Leverage quotations.

  • Transitional Words and Phrases

Revisit the Introduction and Conclusion

  • Grasp the Prompt

Anticipate Counterarguments

Include examples, seek online assistance, read aloud for clarity, 8 ways to make a paper longer, use transitional words and phrases.

  • Utilize “to be” Verbs: Incorporate verbs that start with “to be.” Transform simplistic phrases like “I eat fast” into more descriptive ones like “I am a fast eater.”
  • Add Depth: Avoid brevity. Instead of stating, “We had a good time at the fair,” opt for a more detailed account like “This weekend, my friend and I thoroughly enjoyed our time there. Riding the Ferris wheel and savoring funnel cakes created wonderful memories.”

Meticulously Grasp the Prompt

  • Have you addressed all the questions posed in the prompt?
  • Have you backed up your claims with solid supporting evidence?
  • Are any crucial details missing that could enhance your argument’s clarity?
  • Have you met all the requirements outlined in the prompt besides the length?
  • Have you deleted extra spacing and used the correct margin and font?

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  • Writing a Research Paper
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  • Research Paper Sources
  • Research Paper Problem Statement
  • Research Paper Thesis Statement
  • Hypothesis for a Research Paper
  • Research Question
  • Research Paper Outline
  • Research Paper Summary
  • Research Paper Prospectus
  • Research Paper Proposal
  • Research Paper Format
  • Research Paper Styles
  • AMA Style Research Paper
  • MLA Style Research Paper
  • Chicago Style Research Paper
  • APA Style Research Paper
  • Research Paper Structure
  • Research Paper Cover Page
  • Research Paper Abstract
  • Research Paper Introduction
  • Research Paper Body Paragraph
  • Research Paper Literature Review
  • Research Paper Background
  • Research Paper Methods Section
  • Research Paper Results Section
  • Research Paper Discussion Section
  • Research Paper Conclusion
  • Research Paper Appendix
  • Research Paper Bibliography
  • APA Reference Page
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  • Bibliography vs Works Cited vs References Page
  • Research Paper Types
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21 Helpful and Easy Tips to Make an Essay Longer

Bookman Old Style > Times New Roman.

21 tricks to make an essay longer

When you're writing a school paper after researching and typing for what feels like ages, but you still haven't reached your teacher's required page count, it's normal to feel frustrated. Maybe you get a little creative and play Microsoft Word gymnastics with different fonts and spacing, or become super expressive with your descriptions. There's also a chance you missed something on the assignment rubric, or overlooked the opportunity to include more quotes from trusted sources. You might even be able to load up on a few more examples for your argument, easily boosting the word count with additional research. Still a couple pages behind the limit? Don't worry. Below, we have over 20 tips to help you hit that page requirement.

1. Make sure you included everything on the rubric. If you forgot a whole section focusing on the counter argument, that could be the reason why your paper is a couple pages shorter than needed.

2. Load up on transitional phrases. Your paper isn't long enough, therefore it may be necessary to add some transitional phrases because they take up space. On the other hand , this could make your paper really wordy, however , it may be necessary. See what I did there?

3. Spell out your numbers. There are four editorial styles — AP, APA, MLA, and Chicago. Each one has a different rule for spelling out numbers, which can work out in your favor. For instance, in APA, you write out all numbers under 10. So a one-character "7" becomes a five-character "seven." In MLA, you spell out all numbers at the beginning of a sentence, and all simple numbers (those that are one or two words). Make sure to check the assignment rubric to see what style your paper should be written in!

4. Ditch the contractions. Honestly, you probably should not even be using contractions in a formal essay, so if you are filling up your paper with "don't," "won't," and "can't," switch them out for "do not," "will not," and "cannot."

5. Use numerous examples. Make sure to do extensive research on your essay topic and come up with at least 2-3 examples for every argument presented. One example might seem like enough, but adding a couple more points improves your paper and boosts its word count.

6. Add quotes. Including quotes, whether they be from a book, news article, or trusted source, helps strengthen and validate the point you're making in a paper. But you can't just drop a quotation without context. Introducing, writing out, and properly unpacking a quote can add value — and length — to your essay.

7. Start getting really descriptive about everything. How illustrative can you get about the evolution of electricity, you ask? Well, the answer is: Very. The howling wind gushed passed Benjamin Franklin at 30 miles per hour on that cold, rainy night, pulling the string of his kite taught as it fought to stay in the sky and sent his grey hair flying up in the sky like silvery wisps.

8. Try to make your header longer. If possible, of course. Some teachers clearly state what information needs to be included in the header. But if there's no guideline, add what you can within reason — I'm not sure your teacher is going to appreciate your TikTok or Insta handle listed on the page.

9. Have someone proofread. Getting another pair of eyes to read your paper might reveal some areas in need of work. Maybe you need to elaborate a bit more on a certain argument, or include a quote to strengthen an example.

10. Revisit your introduction paragraph. Sometimes, an introduction is easiest to write after the paper has been written. Having already presented and thoroughly discussed the argument in the essay's body paragraphs, you have a more concrete understanding of what direction the paper takes. There might be some information or ideas you can add into the intro, to better set up the paper's points.

11. Make your spacing larger. Your teacher probably won't be able to tell the difference between double spacing and 2.5 spacing. *fingers crossed*

12 . While you're at it, expand the spacing between the characters. Yes, I'm talking between each and every letter.

13. Raise the font size from 12pt to 12.5pt. Nobody has to know!

14. Make all periods and commas 14pt. It sounds tedious, but simply command-f and search for the period, that way you can change all of them at once.

15. Put extra space around your (super long and bolded) title. It needs some space to shine and breathe, obvs.

16. Change the font. You can't get too crazy or else your teacher will call you out, so you stick with something super similar to Times New Roman, but slightly bigger, like Bookman Old Style. However some teachers specify a certain font in the paper's assignment requirements — in the case, don't try to switch things up.

17. Reverse outline. After you've finished penning your essay, read it through and write an outline on what you have written so far. This strategy can reveal some paragraphs in need of further development. If you notice one super long paragraph, try breaking the ideas down into separate paragraphs. This might bump the page count up a bit, and give you the opportunity to include a few more transition sentences.

18. Make your margins bigger. You have to be careful about the left and right margins, and the top can be tricky. But the bottom margin, you can practically make it as big as you want. And then you can...

19. Add a fancy footer with page numbers. Obviously (hopefully), your teacher will appreciate your attention to detail and presentation.

20. Add a header with the title of your paper to every single page. Just in case your teacher forgets what your paper is about. You only want to help.

21. Make a separate cover page. Technically, the rubric didn't say it couldn't count as page one.

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The Impact of Cognitive Dissonance on Decision-Making: Analyzing Real-World Scenarios

This essay about cognitive dissonance explores its profound influence on decision-making across various real-world contexts. It examines the complex interplay between beliefs, behaviors, and conflicting actions, highlighting how individuals and organizations rationalize decisions and manage the discomfort caused by cognitive dissonance. The text also discusses the broader implications on interpersonal relationships, corporate strategies, and public discourse, emphasizing the importance of recognizing and adapting to cognitive dissonance to improve decision-making processes.

How it works

Cognitive dissonance, appearance psychological charm, gives up profond shadow above confirmation decision in actual well-assorted contexts, creates a prosperous tapestry for research relation and cognition human. This co-operation tangled between trusts, terms, and contradictory actions are served by fat land for a dynamic shrewdness, brings up our alternatives, relation, and in eventual addition, decision results.

Appear he type deeply executes despite the viable dwelling and concession despite a charm spontaneous international vacations, contradicts their principles eco-conscious. This fight between ideals and actions are given up by a cognitive dissonance, conducts despite a variety rails flag devices.

They, at a case, rationalize a decision, distinguishes on a requirement in an interruption or itself calms, that casual condescends no is perceptibly to react an environment. Alternative, they, at a case, searched information, downplaying imprint travel ecological, off-loads they discomfort.

In the corporate world, cognitive dissonance appears often, when strategies no in the state to meet expectations. Weigh a specialist hard-tempered it one is it, contests market access, only, to testify his deserted implementation. Runs into a dissonance between trusts and reality, a specialist, at a case, comes running despite a cognitive gymnastics, to lean a chain, adds abandonment despite external postmen or concentrates he on sedative successes.

However, cognitive dissonance deeply influences interpersonal terms and public conversation. Image political warm discussions, where types a despairing man is protected by their ideological relations. Because one contradictory proof appears, a cognitive dissonance determines defences answers, mixes a structural dialogue and immortalizes polarization. In a public vast context, a cognitive dissonance mixes a compassion and understanding, worsens public divisions and mixes decision for a problem collective efforts. When a chain places he on priorities above a critical estimation, dialogue and collaboration drop, encourages one put on an anchor he relations and vies dynamic.

Recognizing and understands, that cognitive critical dissonance for realization confirmation decision practically. ?ultivating owns and cognitive flexibility can soften his unfavorable things, while creation culture deficit and receptivity despite a feed-back moves adaptive confirmation decision in organizations. So, brings up a compassion and structural dialogue can connect a bridge divides and encourage partners decisions in society. ? conclusion, cognitive cognition man forms dissonance and relation perceptibly. Confirmation his presence and settles complications more inform, adaptive confirmation decision in the personal kingdoms, organizational, and social.

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Cultural Relativity and Acceptance of Embryonic Stem Cell Research

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There is a debate about the ethical implications of using human embryos in stem cell research, which can be influenced by cultural, moral, and social values. This paper argues for an adaptable framework to accommodate diverse cultural and religious perspectives. By using an adaptive ethics model, research protections can reflect various populations and foster growth in stem cell research possibilities.

INTRODUCTION

Stem cell research combines biology, medicine, and technology, promising to alter health care and the understanding of human development. Yet, ethical contention exists because of individuals’ perceptions of using human embryos based on their various cultural, moral, and social values. While these disagreements concerning policy, use, and general acceptance have prompted the development of an international ethics policy, such a uniform approach can overlook the nuanced ethical landscapes between cultures. With diverse viewpoints in public health, a single global policy, especially one reflecting Western ethics or the ethics prevalent in high-income countries, is impractical. This paper argues for a culturally sensitive, adaptable framework for the use of embryonic stem cells. Stem cell policy should accommodate varying ethical viewpoints and promote an effective global dialogue. With an extension of an ethics model that can adapt to various cultures, we recommend localized guidelines that reflect the moral views of the people those guidelines serve.

Stem cells, characterized by their unique ability to differentiate into various cell types, enable the repair or replacement of damaged tissues. Two primary types of stem cells are somatic stem cells (adult stem cells) and embryonic stem cells. Adult stem cells exist in developed tissues and maintain the body’s repair processes. [1] Embryonic stem cells (ESC) are remarkably pluripotent or versatile, making them valuable in research. [2] However, the use of ESCs has sparked ethics debates. Considering the potential of embryonic stem cells, research guidelines are essential. The International Society for Stem Cell Research (ISSCR) provides international stem cell research guidelines. They call for “public conversations touching on the scientific significance as well as the societal and ethical issues raised by ESC research.” [3] The ISSCR also publishes updates about culturing human embryos 14 days post fertilization, suggesting local policies and regulations should continue to evolve as ESC research develops. [4]  Like the ISSCR, which calls for local law and policy to adapt to developing stem cell research given cultural acceptance, this paper highlights the importance of local social factors such as religion and culture.

I.     Global Cultural Perspective of Embryonic Stem Cells

Views on ESCs vary throughout the world. Some countries readily embrace stem cell research and therapies, while others have stricter regulations due to ethical concerns surrounding embryonic stem cells and when an embryo becomes entitled to moral consideration. The philosophical issue of when the “someone” begins to be a human after fertilization, in the morally relevant sense, [5] impacts when an embryo becomes not just worthy of protection but morally entitled to it. The process of creating embryonic stem cell lines involves the destruction of the embryos for research. [6] Consequently, global engagement in ESC research depends on social-cultural acceptability.

a.     US and Rights-Based Cultures

In the United States, attitudes toward stem cell therapies are diverse. The ethics and social approaches, which value individualism, [7] trigger debates regarding the destruction of human embryos, creating a complex regulatory environment. For example, the 1996 Dickey-Wicker Amendment prohibited federal funding for the creation of embryos for research and the destruction of embryos for “more than allowed for research on fetuses in utero.” [8] Following suit, in 2001, the Bush Administration heavily restricted stem cell lines for research. However, the Stem Cell Research Enhancement Act of 2005 was proposed to help develop ESC research but was ultimately vetoed. [9] Under the Obama administration, in 2009, an executive order lifted restrictions allowing for more development in this field. [10] The flux of research capacity and funding parallels the different cultural perceptions of human dignity of the embryo and how it is socially presented within the country’s research culture. [11]

b.     Ubuntu and Collective Cultures

African bioethics differs from Western individualism because of the different traditions and values. African traditions, as described by individuals from South Africa and supported by some studies in other African countries, including Ghana and Kenya, follow the African moral philosophies of Ubuntu or Botho and Ukama , which “advocates for a form of wholeness that comes through one’s relationship and connectedness with other people in the society,” [12] making autonomy a socially collective concept. In this context, for the community to act autonomously, individuals would come together to decide what is best for the collective. Thus, stem cell research would require examining the value of the research to society as a whole and the use of the embryos as a collective societal resource. If society views the source as part of the collective whole, and opposes using stem cells, compromising the cultural values to pursue research may cause social detachment and stunt research growth. [13] Based on local culture and moral philosophy, the permissibility of stem cell research depends on how embryo, stem cell, and cell line therapies relate to the community as a whole . Ubuntu is the expression of humanness, with the person’s identity drawn from the “’I am because we are’” value. [14] The decision in a collectivistic culture becomes one born of cultural context, and individual decisions give deference to others in the society.

Consent differs in cultures where thought and moral philosophy are based on a collective paradigm. So, applying Western bioethical concepts is unrealistic. For one, Africa is a diverse continent with many countries with different belief systems, access to health care, and reliance on traditional or Western medicines. Where traditional medicine is the primary treatment, the “’restrictive focus on biomedically-related bioethics’” [is] problematic in African contexts because it neglects bioethical issues raised by traditional systems.” [15] No single approach applies in all areas or contexts. Rather than evaluating the permissibility of ESC research according to Western concepts such as the four principles approach, different ethics approaches should prevail.

Another consideration is the socio-economic standing of countries. In parts of South Africa, researchers have not focused heavily on contributing to the stem cell discourse, either because it is not considered health care or a health science priority or because resources are unavailable. [16] Each country’s priorities differ given different social, political, and economic factors. In South Africa, for instance, areas such as maternal mortality, non-communicable diseases, telemedicine, and the strength of health systems need improvement and require more focus. [17] Stem cell research could benefit the population, but it also could divert resources from basic medical care. Researchers in South Africa adhere to the National Health Act and Medicines Control Act in South Africa and international guidelines; however, the Act is not strictly enforced, and there is no clear legislation for research conduct or ethical guidelines. [18]

Some parts of Africa condemn stem cell research. For example, 98.2 percent of the Tunisian population is Muslim. [19] Tunisia does not permit stem cell research because of moral conflict with a Fatwa. Religion heavily saturates the regulation and direction of research. [20] Stem cell use became permissible for reproductive purposes only recently, with tight restrictions preventing cells from being used in any research other than procedures concerning ART/IVF.  Their use is conditioned on consent, and available only to married couples. [21] The community's receptiveness to stem cell research depends on including communitarian African ethics.

c.     Asia

Some Asian countries also have a collective model of ethics and decision making. [22] In China, the ethics model promotes a sincere respect for life or human dignity, [23] based on protective medicine. This model, influenced by Traditional Chinese Medicine (TCM), [24] recognizes Qi as the vital energy delivered via the meridians of the body; it connects illness to body systems, the body’s entire constitution, and the universe for a holistic bond of nature, health, and quality of life. [25] Following a protective ethics model, and traditional customs of wholeness, investment in stem cell research is heavily desired for its applications in regenerative therapies, disease modeling, and protective medicines. In a survey of medical students and healthcare practitioners, 30.8 percent considered stem cell research morally unacceptable while 63.5 percent accepted medical research using human embryonic stem cells. Of these individuals, 89.9 percent supported increased funding for stem cell research. [26] The scientific community might not reflect the overall population. From 1997 to 2019, China spent a total of $576 million (USD) on stem cell research at 8,050 stem cell programs, increased published presence from 0.6 percent to 14.01 percent of total global stem cell publications as of 2014, and made significant strides in cell-based therapies for various medical conditions. [27] However, while China has made substantial investments in stem cell research and achieved notable progress in clinical applications, concerns linger regarding ethical oversight and transparency. [28] For example, the China Biosecurity Law, promoted by the National Health Commission and China Hospital Association, attempted to mitigate risks by introducing an institutional review board (IRB) in the regulatory bodies. 5800 IRBs registered with the Chinese Clinical Trial Registry since 2021. [29] However, issues still need to be addressed in implementing effective IRB review and approval procedures.

The substantial government funding and focus on scientific advancement have sometimes overshadowed considerations of regional cultures, ethnic minorities, and individual perspectives, particularly evident during the one-child policy era. As government policy adapts to promote public stability, such as the change from the one-child to the two-child policy, [30] research ethics should also adapt to ensure respect for the values of its represented peoples.

Japan is also relatively supportive of stem cell research and therapies. Japan has a more transparent regulatory framework, allowing for faster approval of regenerative medicine products, which has led to several advanced clinical trials and therapies. [31] South Korea is also actively engaged in stem cell research and has a history of breakthroughs in cloning and embryonic stem cells. [32] However, the field is controversial, and there are issues of scientific integrity. For example, the Korean FDA fast-tracked products for approval, [33] and in another instance, the oocyte source was unclear and possibly violated ethical standards. [34] Trust is important in research, as it builds collaborative foundations between colleagues, trial participant comfort, open-mindedness for complicated and sensitive discussions, and supports regulatory procedures for stakeholders. There is a need to respect the culture’s interest, engagement, and for research and clinical trials to be transparent and have ethical oversight to promote global research discourse and trust.

d.     Middle East

Countries in the Middle East have varying degrees of acceptance of or restrictions to policies related to using embryonic stem cells due to cultural and religious influences. Saudi Arabia has made significant contributions to stem cell research, and conducts research based on international guidelines for ethical conduct and under strict adherence to guidelines in accordance with Islamic principles. Specifically, the Saudi government and people require ESC research to adhere to Sharia law. In addition to umbilical and placental stem cells, [35] Saudi Arabia permits the use of embryonic stem cells as long as they come from miscarriages, therapeutic abortions permissible by Sharia law, or are left over from in vitro fertilization and donated to research. [36] Laws and ethical guidelines for stem cell research allow the development of research institutions such as the King Abdullah International Medical Research Center, which has a cord blood bank and a stem cell registry with nearly 10,000 donors. [37] Such volume and acceptance are due to the ethical ‘permissibility’ of the donor sources, which do not conflict with religious pillars. However, some researchers err on the side of caution, choosing not to use embryos or fetal tissue as they feel it is unethical to do so. [38]

Jordan has a positive research ethics culture. [39] However, there is a significant issue of lack of trust in researchers, with 45.23 percent (38.66 percent agreeing and 6.57 percent strongly agreeing) of Jordanians holding a low level of trust in researchers, compared to 81.34 percent of Jordanians agreeing that they feel safe to participate in a research trial. [40] Safety testifies to the feeling of confidence that adequate measures are in place to protect participants from harm, whereas trust in researchers could represent the confidence in researchers to act in the participants’ best interests, adhere to ethical guidelines, provide accurate information, and respect participants’ rights and dignity. One method to improve trust would be to address communication issues relevant to ESC. Legislation surrounding stem cell research has adopted specific language, especially concerning clarification “between ‘stem cells’ and ‘embryonic stem cells’” in translation. [41] Furthermore, legislation “mandates the creation of a national committee… laying out specific regulations for stem-cell banking in accordance with international standards.” [42] This broad regulation opens the door for future global engagement and maintains transparency. However, these regulations may also constrain the influence of research direction, pace, and accessibility of research outcomes.

e.     Europe

In the European Union (EU), ethics is also principle-based, but the principles of autonomy, dignity, integrity, and vulnerability are interconnected. [43] As such, the opportunity for cohesion and concessions between individuals’ thoughts and ideals allows for a more adaptable ethics model due to the flexible principles that relate to the human experience The EU has put forth a framework in its Convention for the Protection of Human Rights and Dignity of the Human Being allowing member states to take different approaches. Each European state applies these principles to its specific conventions, leading to or reflecting different acceptance levels of stem cell research. [44]

For example, in Germany, Lebenzusammenhang , or the coherence of life, references integrity in the unity of human culture. Namely, the personal sphere “should not be subject to external intervention.” [45]  Stem cell interventions could affect this concept of bodily completeness, leading to heavy restrictions. Under the Grundgesetz, human dignity and the right to life with physical integrity are paramount. [46] The Embryo Protection Act of 1991 made producing cell lines illegal. Cell lines can be imported if approved by the Central Ethics Commission for Stem Cell Research only if they were derived before May 2007. [47] Stem cell research respects the integrity of life for the embryo with heavy specifications and intense oversight. This is vastly different in Finland, where the regulatory bodies find research more permissible in IVF excess, but only up to 14 days after fertilization. [48] Spain’s approach differs still, with a comprehensive regulatory framework. [49] Thus, research regulation can be culture-specific due to variations in applied principles. Diverse cultures call for various approaches to ethical permissibility. [50] Only an adaptive-deliberative model can address the cultural constructions of self and achieve positive, culturally sensitive stem cell research practices. [51]

II.     Religious Perspectives on ESC

Embryonic stem cell sources are the main consideration within religious contexts. While individuals may not regard their own religious texts as authoritative or factual, religion can shape their foundations or perspectives.

The Qur'an states:

“And indeed We created man from a quintessence of clay. Then We placed within him a small quantity of nutfa (sperm to fertilize) in a safe place. Then We have fashioned the nutfa into an ‘alaqa (clinging clot or cell cluster), then We developed the ‘alaqa into mudgha (a lump of flesh), and We made mudgha into bones, and clothed the bones with flesh, then We brought it into being as a new creation. So Blessed is Allah, the Best of Creators.” [52]

Many scholars of Islam estimate the time of soul installment, marked by the angel breathing in the soul to bring the individual into creation, as 120 days from conception. [53] Personhood begins at this point, and the value of life would prohibit research or experimentation that could harm the individual. If the fetus is more than 120 days old, the time ensoulment is interpreted to occur according to Islamic law, abortion is no longer permissible. [54] There are a few opposing opinions about early embryos in Islamic traditions. According to some Islamic theologians, there is no ensoulment of the early embryo, which is the source of stem cells for ESC research. [55]

In Buddhism, the stance on stem cell research is not settled. The main tenets, the prohibition against harming or destroying others (ahimsa) and the pursuit of knowledge (prajña) and compassion (karuna), leave Buddhist scholars and communities divided. [56] Some scholars argue stem cell research is in accordance with the Buddhist tenet of seeking knowledge and ending human suffering. Others feel it violates the principle of not harming others. Finding the balance between these two points relies on the karmic burden of Buddhist morality. In trying to prevent ahimsa towards the embryo, Buddhist scholars suggest that to comply with Buddhist tenets, research cannot be done as the embryo has personhood at the moment of conception and would reincarnate immediately, harming the individual's ability to build their karmic burden. [57] On the other hand, the Bodhisattvas, those considered to be on the path to enlightenment or Nirvana, have given organs and flesh to others to help alleviate grieving and to benefit all. [58] Acceptance varies on applied beliefs and interpretations.

Catholicism does not support embryonic stem cell research, as it entails creation or destruction of human embryos. This destruction conflicts with the belief in the sanctity of life. For example, in the Old Testament, Genesis describes humanity as being created in God’s image and multiplying on the Earth, referencing the sacred rights to human conception and the purpose of development and life. In the Ten Commandments, the tenet that one should not kill has numerous interpretations where killing could mean murder or shedding of the sanctity of life, demonstrating the high value of human personhood. In other books, the theological conception of when life begins is interpreted as in utero, [59] highlighting the inviolability of life and its formation in vivo to make a religious point for accepting such research as relatively limited, if at all. [60] The Vatican has released ethical directives to help apply a theological basis to modern-day conflicts. The Magisterium of the Church states that “unless there is a moral certainty of not causing harm,” experimentation on fetuses, fertilized cells, stem cells, or embryos constitutes a crime. [61] Such procedures would not respect the human person who exists at these stages, according to Catholicism. Damages to the embryo are considered gravely immoral and illicit. [62] Although the Catholic Church officially opposes abortion, surveys demonstrate that many Catholic people hold pro-choice views, whether due to the context of conception, stage of pregnancy, threat to the mother’s life, or for other reasons, demonstrating that practicing members can also accept some but not all tenets. [63]

Some major Jewish denominations, such as the Reform, Conservative, and Reconstructionist movements, are open to supporting ESC use or research as long as it is for saving a life. [64] Within Judaism, the Talmud, or study, gives personhood to the child at birth and emphasizes that life does not begin at conception: [65]

“If she is found pregnant, until the fortieth day it is mere fluid,” [66]

Whereas most religions prioritize the status of human embryos, the Halakah (Jewish religious law) states that to save one life, most other religious laws can be ignored because it is in pursuit of preservation. [67] Stem cell research is accepted due to application of these religious laws.

We recognize that all religions contain subsets and sects. The variety of environmental and cultural differences within religious groups requires further analysis to respect the flexibility of religious thoughts and practices. We make no presumptions that all cultures require notions of autonomy or morality as under the common morality theory , which asserts a set of universal moral norms that all individuals share provides moral reasoning and guides ethical decisions. [68] We only wish to show that the interaction with morality varies between cultures and countries.

III.     A Flexible Ethical Approach

The plurality of different moral approaches described above demonstrates that there can be no universally acceptable uniform law for ESC on a global scale. Instead of developing one standard, flexible ethical applications must be continued. We recommend local guidelines that incorporate important cultural and ethical priorities.

While the Declaration of Helsinki is more relevant to people in clinical trials receiving ESC products, in keeping with the tradition of protections for research subjects, consent of the donor is an ethical requirement for ESC donation in many jurisdictions including the US, Canada, and Europe. [69] The Declaration of Helsinki provides a reference point for regulatory standards and could potentially be used as a universal baseline for obtaining consent prior to gamete or embryo donation.

For instance, in Columbia University’s egg donor program for stem cell research, donors followed standard screening protocols and “underwent counseling sessions that included information as to the purpose of oocyte donation for research, what the oocytes would be used for, the risks and benefits of donation, and process of oocyte stimulation” to ensure transparency for consent. [70] The program helped advance stem cell research and provided clear and safe research methods with paid participants. Though paid participation or covering costs of incidental expenses may not be socially acceptable in every culture or context, [71] and creating embryos for ESC research is illegal in many jurisdictions, Columbia’s program was effective because of the clear and honest communications with donors, IRBs, and related stakeholders.  This example demonstrates that cultural acceptance of scientific research and of the idea that an egg or embryo does not have personhood is likely behind societal acceptance of donating eggs for ESC research. As noted, many countries do not permit the creation of embryos for research.

Proper communication and education regarding the process and purpose of stem cell research may bolster comprehension and garner more acceptance. “Given the sensitive subject material, a complete consent process can support voluntary participation through trust, understanding, and ethical norms from the cultures and morals participants value. This can be hard for researchers entering countries of different socioeconomic stability, with different languages and different societal values. [72]

An adequate moral foundation in medical ethics is derived from the cultural and religious basis that informs knowledge and actions. [73] Understanding local cultural and religious values and their impact on research could help researchers develop humility and promote inclusion.

IV.     Concerns

Some may argue that if researchers all adhere to one ethics standard, protection will be satisfied across all borders, and the global public will trust researchers. However, defining what needs to be protected and how to define such research standards is very specific to the people to which standards are applied. We suggest that applying one uniform guide cannot accurately protect each individual because we all possess our own perceptions and interpretations of social values. [74] Therefore, the issue of not adjusting to the moral pluralism between peoples in applying one standard of ethics can be resolved by building out ethics models that can be adapted to different cultures and religions.

Other concerns include medical tourism, which may promote health inequities. [75] Some countries may develop and approve products derived from ESC research before others, compromising research ethics or drug approval processes. There are also concerns about the sale of unauthorized stem cell treatments, for example, those without FDA approval in the United States. Countries with robust research infrastructures may be tempted to attract medical tourists, and some customers will have false hopes based on aggressive publicity of unproven treatments. [76]

For example, in China, stem cell clinics can market to foreign clients who are not protected under the regulatory regimes. Companies employ a marketing strategy of “ethically friendly” therapies. Specifically, in the case of Beike, China’s leading stem cell tourism company and sprouting network, ethical oversight of administrators or health bureaus at one site has “the unintended consequence of shifting questionable activities to another node in Beike's diffuse network.” [77] In contrast, Jordan is aware of stem cell research’s potential abuse and its own status as a “health-care hub.” Jordan’s expanded regulations include preserving the interests of individuals in clinical trials and banning private companies from ESC research to preserve transparency and the integrity of research practices. [78]

The social priorities of the community are also a concern. The ISSCR explicitly states that guidelines “should be periodically revised to accommodate scientific advances, new challenges, and evolving social priorities.” [79] The adaptable ethics model extends this consideration further by addressing whether research is warranted given the varying degrees of socioeconomic conditions, political stability, and healthcare accessibilities and limitations. An ethical approach would require discussion about resource allocation and appropriate distribution of funds. [80]

While some religions emphasize the sanctity of life from conception, which may lead to public opposition to ESC research, others encourage ESC research due to its potential for healing and alleviating human pain. Many countries have special regulations that balance local views on embryonic personhood, the benefits of research as individual or societal goods, and the protection of human research subjects. To foster understanding and constructive dialogue, global policy frameworks should prioritize the protection of universal human rights, transparency, and informed consent. In addition to these foundational global policies, we recommend tailoring local guidelines to reflect the diverse cultural and religious perspectives of the populations they govern. Ethics models should be adapted to local populations to effectively establish research protections, growth, and possibilities of stem cell research.

For example, in countries with strong beliefs in the moral sanctity of embryos or heavy religious restrictions, an adaptive model can allow for discussion instead of immediate rejection. In countries with limited individual rights and voice in science policy, an adaptive model ensures cultural, moral, and religious views are taken into consideration, thereby building social inclusion. While this ethical consideration by the government may not give a complete voice to every individual, it will help balance policies and maintain the diverse perspectives of those it affects. Embracing an adaptive ethics model of ESC research promotes open-minded dialogue and respect for the importance of human belief and tradition. By actively engaging with cultural and religious values, researchers can better handle disagreements and promote ethical research practices that benefit each society.

This brief exploration of the religious and cultural differences that impact ESC research reveals the nuances of relative ethics and highlights a need for local policymakers to apply a more intense adaptive model.

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[5] Concerning the moral philosophies of stem cell research, our paper does not posit a personal moral stance nor delve into the “when” of human life begins. To read further about the philosophical debate, consider the following sources:

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[7] Socially, at its core, the Western approach to ethics is widely principle-based, autonomy being one of the key factors to ensure a fundamental respect for persons within research. For information regarding autonomy in research, see: Department of Health, Education, and Welfare, & National Commission for the Protection of Human Subjects of Biomedical and Behavioral Research (1978). The Belmont Report. Ethical principles and guidelines for the protection of human subjects of research.; For a more in-depth review of autonomy within the US, see: Beauchamp, T. L., & Childress, J. F. (1994). Principles of Biomedical Ethics . Oxford University Press.

[8] Sherley v. Sebelius , 644 F.3d 388 (D.C. Cir. 2011), citing 45 C.F.R. 46.204(b) and [42 U.S.C. § 289g(b)]. https://www.cadc.uscourts.gov/internet/opinions.nsf/6c690438a9b43dd685257a64004ebf99/$file/11-5241-1391178.pdf

[9] Stem Cell Research Enhancement Act of 2005, H. R. 810, 109 th Cong. (2001). https://www.govtrack.us/congress/bills/109/hr810/text ; Bush, G. W. (2006, July 19). Message to the House of Representatives . National Archives and Records Administration. https://georgewbush-whitehouse.archives.gov/news/releases/2006/07/20060719-5.html

[10] National Archives and Records Administration. (2009, March 9). Executive order 13505 -- removing barriers to responsible scientific research involving human stem cells . National Archives and Records Administration. https://obamawhitehouse.archives.gov/the-press-office/removing-barriers-responsible-scientific-research-involving-human-stem-cells

[11] Hurlbut, W. B. (2006). Science, Religion, and the Politics of Stem Cells.  Social Research ,  73 (3), 819–834. http://www.jstor.org/stable/40971854

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[13] Source for further reading: Tangwa G. B. (2007). Moral status of embryonic stem cells: perspective of an African villager. Bioethics , 21(8), 449–457. https://doi.org/10.1111/j.1467-8519.2007.00582.x , see also Mnisi, F. M. (2020). An African analysis based on ethics of Ubuntu - are human embryonic stem cell patents morally justifiable? African Insight , 49 (4).

[14] Jecker, N. S., & Atuire, C. (2021). Bioethics in Africa: A contextually enlightened analysis of three cases. Developing World Bioethics , 22 (2), 112–122. https://doi.org/10.1111/dewb.12324

[15] Jecker, N. S., & Atuire, C. (2021). Bioethics in Africa: A contextually enlightened analysis of three cases. Developing World Bioethics, 22(2), 112–122. https://doi.org/10.1111/dewb.12324

[16] Jackson, C.S., Pepper, M.S. Opportunities and barriers to establishing a cell therapy programme in South Africa.  Stem Cell Res Ther   4 , 54 (2013). https://doi.org/10.1186/scrt204 ; Pew Research Center. (2014, May 1). Public health a major priority in African nations . Pew Research Center’s Global Attitudes Project. https://www.pewresearch.org/global/2014/05/01/public-health-a-major-priority-in-african-nations/

[17] Department of Health Republic of South Africa. (2021). Health Research Priorities (revised) for South Africa 2021-2024 . National Health Research Strategy. https://www.health.gov.za/wp-content/uploads/2022/05/National-Health-Research-Priorities-2021-2024.pdf

[18] Oosthuizen, H. (2013). Legal and Ethical Issues in Stem Cell Research in South Africa. In: Beran, R. (eds) Legal and Forensic Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32338-6_80 , see also: Gaobotse G (2018) Stem Cell Research in Africa: Legislation and Challenges. J Regen Med 7:1. doi: 10.4172/2325-9620.1000142

[19] United States Bureau of Citizenship and Immigration Services. (1998). Tunisia: Information on the status of Christian conversions in Tunisia . UNHCR Web Archive. https://webarchive.archive.unhcr.org/20230522142618/https://www.refworld.org/docid/3df0be9a2.html

[20] Gaobotse, G. (2018) Stem Cell Research in Africa: Legislation and Challenges. J Regen Med 7:1. doi: 10.4172/2325-9620.1000142

[21] Kooli, C. Review of assisted reproduction techniques, laws, and regulations in Muslim countries.  Middle East Fertil Soc J   24 , 8 (2020). https://doi.org/10.1186/s43043-019-0011-0 ; Gaobotse, G. (2018) Stem Cell Research in Africa: Legislation and Challenges. J Regen Med 7:1. doi: 10.4172/2325-9620.1000142

[22] Pang M. C. (1999). Protective truthfulness: the Chinese way of safeguarding patients in informed treatment decisions. Journal of medical ethics , 25(3), 247–253. https://doi.org/10.1136/jme.25.3.247

[23] Wang, L., Wang, F., & Zhang, W. (2021). Bioethics in China’s biosecurity law: Forms, effects, and unsettled issues. Journal of law and the biosciences , 8(1).  https://doi.org/10.1093/jlb/lsab019 https://academic.oup.com/jlb/article/8/1/lsab019/6299199

[24] Wang, Y., Xue, Y., & Guo, H. D. (2022). Intervention effects of traditional Chinese medicine on stem cell therapy of myocardial infarction.  Frontiers in pharmacology ,  13 , 1013740. https://doi.org/10.3389/fphar.2022.1013740

[25] Li, X.-T., & Zhao, J. (2012). Chapter 4: An Approach to the Nature of Qi in TCM- Qi and Bioenergy. In Recent Advances in Theories and Practice of Chinese Medicine (p. 79). InTech.

[26] Luo, D., Xu, Z., Wang, Z., & Ran, W. (2021). China's Stem Cell Research and Knowledge Levels of Medical Practitioners and Students.  Stem cells international ,  2021 , 6667743. https://doi.org/10.1155/2021/6667743

[27] Luo, D., Xu, Z., Wang, Z., & Ran, W. (2021). China's Stem Cell Research and Knowledge Levels of Medical Practitioners and Students.  Stem cells international ,  2021 , 6667743. https://doi.org/10.1155/2021/6667743

[28] Zhang, J. Y. (2017). Lost in translation? accountability and governance of Clinical Stem Cell Research in China. Regenerative Medicine , 12 (6), 647–656. https://doi.org/10.2217/rme-2017-0035

[29] Wang, L., Wang, F., & Zhang, W. (2021). Bioethics in China’s biosecurity law: Forms, effects, and unsettled issues. Journal of law and the biosciences , 8(1).  https://doi.org/10.1093/jlb/lsab019 https://academic.oup.com/jlb/article/8/1/lsab019/6299199

[30] Chen, H., Wei, T., Wang, H.  et al.  Association of China’s two-child policy with changes in number of births and birth defects rate, 2008–2017.  BMC Public Health   22 , 434 (2022). https://doi.org/10.1186/s12889-022-12839-0

[31] Azuma, K. Regulatory Landscape of Regenerative Medicine in Japan.  Curr Stem Cell Rep   1 , 118–128 (2015). https://doi.org/10.1007/s40778-015-0012-6

[32] Harris, R. (2005, May 19). Researchers Report Advance in Stem Cell Production . NPR. https://www.npr.org/2005/05/19/4658967/researchers-report-advance-in-stem-cell-production

[33] Park, S. (2012). South Korea steps up stem-cell work.  Nature . https://doi.org/10.1038/nature.2012.10565

[34] Resnik, D. B., Shamoo, A. E., & Krimsky, S. (2006). Fraudulent human embryonic stem cell research in South Korea: lessons learned.  Accountability in research ,  13 (1), 101–109. https://doi.org/10.1080/08989620600634193 .

[35] Alahmad, G., Aljohani, S., & Najjar, M. F. (2020). Ethical challenges regarding the use of stem cells: interviews with researchers from Saudi Arabia. BMC medical ethics, 21(1), 35. https://doi.org/10.1186/s12910-020-00482-6

[36] Association for the Advancement of Blood and Biotherapies.  https://www.aabb.org/regulatory-and-advocacy/regulatory-affairs/regulatory-for-cellular-therapies/international-competent-authorities/saudi-arabia

[37] Alahmad, G., Aljohani, S., & Najjar, M. F. (2020). Ethical challenges regarding the use of stem cells: Interviews with researchers from Saudi Arabia.  BMC medical ethics ,  21 (1), 35. https://doi.org/10.1186/s12910-020-00482-6

[38] Alahmad, G., Aljohani, S., & Najjar, M. F. (2020). Ethical challenges regarding the use of stem cells: Interviews with researchers from Saudi Arabia. BMC medical ethics , 21(1), 35. https://doi.org/10.1186/s12910-020-00482-6

Culturally, autonomy practices follow a relational autonomy approach based on a paternalistic deontological health care model. The adherence to strict international research policies and religious pillars within the regulatory environment is a great foundation for research ethics. However, there is a need to develop locally targeted ethics approaches for research (as called for in Alahmad, G., Aljohani, S., & Najjar, M. F. (2020). Ethical challenges regarding the use of stem cells: interviews with researchers from Saudi Arabia. BMC medical ethics, 21(1), 35. https://doi.org/10.1186/s12910-020-00482-6), this decision-making approach may help advise a research decision model. For more on the clinical cultural autonomy approaches, see: Alabdullah, Y. Y., Alzaid, E., Alsaad, S., Alamri, T., Alolayan, S. W., Bah, S., & Aljoudi, A. S. (2022). Autonomy and paternalism in Shared decision‐making in a Saudi Arabian tertiary hospital: A cross‐sectional study. Developing World Bioethics , 23 (3), 260–268. https://doi.org/10.1111/dewb.12355 ; Bukhari, A. A. (2017). Universal Principles of Bioethics and Patient Rights in Saudi Arabia (Doctoral dissertation, Duquesne University). https://dsc.duq.edu/etd/124; Ladha, S., Nakshawani, S. A., Alzaidy, A., & Tarab, B. (2023, October 26). Islam and Bioethics: What We All Need to Know . Columbia University School of Professional Studies. https://sps.columbia.edu/events/islam-and-bioethics-what-we-all-need-know

[39] Ababneh, M. A., Al-Azzam, S. I., Alzoubi, K., Rababa’h, A., & Al Demour, S. (2021). Understanding and attitudes of the Jordanian public about clinical research ethics.  Research Ethics ,  17 (2), 228-241.  https://doi.org/10.1177/1747016120966779

[40] Ababneh, M. A., Al-Azzam, S. I., Alzoubi, K., Rababa’h, A., & Al Demour, S. (2021). Understanding and attitudes of the Jordanian public about clinical research ethics.  Research Ethics ,  17 (2), 228-241.  https://doi.org/10.1177/1747016120966779

[41] Dajani, R. (2014). Jordan’s stem-cell law can guide the Middle East.  Nature  510, 189. https://doi.org/10.1038/510189a

[42] Dajani, R. (2014). Jordan’s stem-cell law can guide the Middle East.  Nature  510, 189. https://doi.org/10.1038/510189a

[43] The EU’s definition of autonomy relates to the capacity for creating ideas, moral insight, decisions, and actions without constraint, personal responsibility, and informed consent. However, the EU views autonomy as not completely able to protect individuals and depends on other principles, such as dignity, which “expresses the intrinsic worth and fundamental equality of all human beings.” Rendtorff, J.D., Kemp, P. (2019). Four Ethical Principles in European Bioethics and Biolaw: Autonomy, Dignity, Integrity and Vulnerability. In: Valdés, E., Lecaros, J. (eds) Biolaw and Policy in the Twenty-First Century. International Library of Ethics, Law, and the New Medicine, vol 78. Springer, Cham. https://doi.org/10.1007/978-3-030-05903-3_3

[44] Council of Europe. Convention for the protection of Human Rights and Dignity of the Human Being with regard to the Application of Biology and Medicine: Convention on Human Rights and Biomedicine (ETS No. 164) https://www.coe.int/en/web/conventions/full-list?module=treaty-detail&treatynum=164 (forbidding the creation of embryos for research purposes only, and suggests embryos in vitro have protections.); Also see Drabiak-Syed B. K. (2013). New President, New Human Embryonic Stem Cell Research Policy: Comparative International Perspectives and Embryonic Stem Cell Research Laws in France.  Biotechnology Law Report ,  32 (6), 349–356. https://doi.org/10.1089/blr.2013.9865

[45] Rendtorff, J.D., Kemp, P. (2019). Four Ethical Principles in European Bioethics and Biolaw: Autonomy, Dignity, Integrity and Vulnerability. In: Valdés, E., Lecaros, J. (eds) Biolaw and Policy in the Twenty-First Century. International Library of Ethics, Law, and the New Medicine, vol 78. Springer, Cham. https://doi.org/10.1007/978-3-030-05903-3_3

[46] Tomuschat, C., Currie, D. P., Kommers, D. P., & Kerr, R. (Trans.). (1949, May 23). Basic law for the Federal Republic of Germany. https://www.btg-bestellservice.de/pdf/80201000.pdf

[47] Regulation of Stem Cell Research in Germany . Eurostemcell. (2017, April 26). https://www.eurostemcell.org/regulation-stem-cell-research-germany

[48] Regulation of Stem Cell Research in Finland . Eurostemcell. (2017, April 26). https://www.eurostemcell.org/regulation-stem-cell-research-finland

[49] Regulation of Stem Cell Research in Spain . Eurostemcell. (2017, April 26). https://www.eurostemcell.org/regulation-stem-cell-research-spain

[50] Some sources to consider regarding ethics models or regulatory oversights of other cultures not covered:

Kara MA. Applicability of the principle of respect for autonomy: the perspective of Turkey. J Med Ethics. 2007 Nov;33(11):627-30. doi: 10.1136/jme.2006.017400. PMID: 17971462; PMCID: PMC2598110.

Ugarte, O. N., & Acioly, M. A. (2014). The principle of autonomy in Brazil: one needs to discuss it ...  Revista do Colegio Brasileiro de Cirurgioes ,  41 (5), 374–377. https://doi.org/10.1590/0100-69912014005013

Bharadwaj, A., & Glasner, P. E. (2012). Local cells, global science: The rise of embryonic stem cell research in India . Routledge.

For further research on specific European countries regarding ethical and regulatory framework, we recommend this database: Regulation of Stem Cell Research in Europe . Eurostemcell. (2017, April 26). https://www.eurostemcell.org/regulation-stem-cell-research-europe   

[51] Klitzman, R. (2006). Complications of culture in obtaining informed consent. The American Journal of Bioethics, 6(1), 20–21. https://doi.org/10.1080/15265160500394671 see also: Ekmekci, P. E., & Arda, B. (2017). Interculturalism and Informed Consent: Respecting Cultural Differences without Breaching Human Rights.  Cultura (Iasi, Romania) ,  14 (2), 159–172.; For why trust is important in research, see also: Gray, B., Hilder, J., Macdonald, L., Tester, R., Dowell, A., & Stubbe, M. (2017). Are research ethics guidelines culturally competent?  Research Ethics ,  13 (1), 23-41.  https://doi.org/10.1177/1747016116650235

[52] The Qur'an  (M. Khattab, Trans.). (1965). Al-Mu’minun, 23: 12-14. https://quran.com/23

[53] Lenfest, Y. (2017, December 8). Islam and the beginning of human life . Bill of Health. https://blog.petrieflom.law.harvard.edu/2017/12/08/islam-and-the-beginning-of-human-life/

[54] Aksoy, S. (2005). Making regulations and drawing up legislation in Islamic countries under conditions of uncertainty, with special reference to embryonic stem cell research. Journal of Medical Ethics , 31: 399-403.; see also: Mahmoud, Azza. "Islamic Bioethics: National Regulations and Guidelines of Human Stem Cell Research in the Muslim World." Master's thesis, Chapman University, 2022. https://doi.org/10.36837/ chapman.000386

[55] Rashid, R. (2022). When does Ensoulment occur in the Human Foetus. Journal of the British Islamic Medical Association , 12 (4). ISSN 2634 8071. https://www.jbima.com/wp-content/uploads/2023/01/2-Ethics-3_-Ensoulment_Rafaqat.pdf.

[56] Sivaraman, M. & Noor, S. (2017). Ethics of embryonic stem cell research according to Buddhist, Hindu, Catholic, and Islamic religions: perspective from Malaysia. Asian Biomedicine,8(1) 43-52.  https://doi.org/10.5372/1905-7415.0801.260

[57] Jafari, M., Elahi, F., Ozyurt, S. & Wrigley, T. (2007). 4. Religious Perspectives on Embryonic Stem Cell Research. In K. Monroe, R. Miller & J. Tobis (Ed.),  Fundamentals of the Stem Cell Debate: The Scientific, Religious, Ethical, and Political Issues  (pp. 79-94). Berkeley: University of California Press.  https://escholarship.org/content/qt9rj0k7s3/qt9rj0k7s3_noSplash_f9aca2e02c3777c7fb76ea768ba458f0.pdf https://doi.org/10.1525/9780520940994-005

[58] Lecso, P. A. (1991). The Bodhisattva Ideal and Organ Transplantation.  Journal of Religion and Health ,  30 (1), 35–41. http://www.jstor.org/stable/27510629 ; Bodhisattva, S. (n.d.). The Key of Becoming a Bodhisattva . A Guide to the Bodhisattva Way of Life. http://www.buddhism.org/Sutras/2/BodhisattvaWay.htm

[59] There is no explicit religious reference to when life begins or how to conduct research that interacts with the concept of life. However, these are relevant verses pertaining to how the fetus is viewed. (( King James Bible . (1999). Oxford University Press. (original work published 1769))

Jerimiah 1: 5 “Before I formed thee in the belly I knew thee; and before thou camest forth out of the womb I sanctified thee…”

In prophet Jerimiah’s insight, God set him apart as a person known before childbirth, a theme carried within the Psalm of David.

Psalm 139: 13-14 “…Thou hast covered me in my mother's womb. I will praise thee; for I am fearfully and wonderfully made…”

These verses demonstrate David’s respect for God as an entity that would know of all man’s thoughts and doings even before birth.

[60] It should be noted that abortion is not supported as well.

[61] The Vatican. (1987, February 22). Instruction on Respect for Human Life in Its Origin and on the Dignity of Procreation Replies to Certain Questions of the Day . Congregation For the Doctrine of the Faith. https://www.vatican.va/roman_curia/congregations/cfaith/documents/rc_con_cfaith_doc_19870222_respect-for-human-life_en.html

[62] The Vatican. (2000, August 25). Declaration On the Production and the Scientific and Therapeutic Use of Human Embryonic Stem Cells . Pontifical Academy for Life. https://www.vatican.va/roman_curia/pontifical_academies/acdlife/documents/rc_pa_acdlife_doc_20000824_cellule-staminali_en.html ; Ohara, N. (2003). Ethical Consideration of Experimentation Using Living Human Embryos: The Catholic Church’s Position on Human Embryonic Stem Cell Research and Human Cloning. Department of Obstetrics and Gynecology . Retrieved from https://article.imrpress.com/journal/CEOG/30/2-3/pii/2003018/77-81.pdf.

[63] Smith, G. A. (2022, May 23). Like Americans overall, Catholics vary in their abortion views, with regular mass attenders most opposed . Pew Research Center. https://www.pewresearch.org/short-reads/2022/05/23/like-americans-overall-catholics-vary-in-their-abortion-views-with-regular-mass-attenders-most-opposed/

[64] Rosner, F., & Reichman, E. (2002). Embryonic stem cell research in Jewish law. Journal of halacha and contemporary society , (43), 49–68.; Jafari, M., Elahi, F., Ozyurt, S. & Wrigley, T. (2007). 4. Religious Perspectives on Embryonic Stem Cell Research. In K. Monroe, R. Miller & J. Tobis (Ed.),  Fundamentals of the Stem Cell Debate: The Scientific, Religious, Ethical, and Political Issues  (pp. 79-94). Berkeley: University of California Press.  https://escholarship.org/content/qt9rj0k7s3/qt9rj0k7s3_noSplash_f9aca2e02c3777c7fb76ea768ba458f0.pdf https://doi.org/10.1525/9780520940994-005

[65] Schenker J. G. (2008). The beginning of human life: status of embryo. Perspectives in Halakha (Jewish Religious Law).  Journal of assisted reproduction and genetics ,  25 (6), 271–276. https://doi.org/10.1007/s10815-008-9221-6

[66] Ruttenberg, D. (2020, May 5). The Torah of Abortion Justice (annotated source sheet) . Sefaria. https://www.sefaria.org/sheets/234926.7?lang=bi&with=all&lang2=en

[67] Jafari, M., Elahi, F., Ozyurt, S. & Wrigley, T. (2007). 4. Religious Perspectives on Embryonic Stem Cell Research. In K. Monroe, R. Miller & J. Tobis (Ed.),  Fundamentals of the Stem Cell Debate: The Scientific, Religious, Ethical, and Political Issues  (pp. 79-94). Berkeley: University of California Press.  https://escholarship.org/content/qt9rj0k7s3/qt9rj0k7s3_noSplash_f9aca2e02c3777c7fb76ea768ba458f0.pdf https://doi.org/10.1525/9780520940994-005

[68] Gert, B. (2007). Common morality: Deciding what to do . Oxford Univ. Press.

[69] World Medical Association (2013). World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA , 310(20), 2191–2194. https://doi.org/10.1001/jama.2013.281053 Declaration of Helsinki – WMA – The World Medical Association .; see also: National Commission for the Protection of Human Subjects of Biomedical and Behavioral Research. (1979).  The Belmont report: Ethical principles and guidelines for the protection of human subjects of research . U.S. Department of Health and Human Services.  https://www.hhs.gov/ohrp/regulations-and-policy/belmont-report/read-the-belmont-report/index.html

[70] Zakarin Safier, L., Gumer, A., Kline, M., Egli, D., & Sauer, M. V. (2018). Compensating human subjects providing oocytes for stem cell research: 9-year experience and outcomes.  Journal of assisted reproduction and genetics ,  35 (7), 1219–1225. https://doi.org/10.1007/s10815-018-1171-z https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063839/ see also: Riordan, N. H., & Paz Rodríguez, J. (2021). Addressing concerns regarding associated costs, transparency, and integrity of research in recent stem cell trial. Stem Cells Translational Medicine , 10 (12), 1715–1716. https://doi.org/10.1002/sctm.21-0234

[71] Klitzman, R., & Sauer, M. V. (2009). Payment of egg donors in stem cell research in the USA.  Reproductive biomedicine online ,  18 (5), 603–608. https://doi.org/10.1016/s1472-6483(10)60002-8

[72] Krosin, M. T., Klitzman, R., Levin, B., Cheng, J., & Ranney, M. L. (2006). Problems in comprehension of informed consent in rural and peri-urban Mali, West Africa.  Clinical trials (London, England) ,  3 (3), 306–313. https://doi.org/10.1191/1740774506cn150oa

[73] Veatch, Robert M.  Hippocratic, Religious, and Secular Medical Ethics: The Points of Conflict . Georgetown University Press, 2012.

[74] Msoroka, M. S., & Amundsen, D. (2018). One size fits not quite all: Universal research ethics with diversity.  Research Ethics ,  14 (3), 1-17.  https://doi.org/10.1177/1747016117739939

[75] Pirzada, N. (2022). The Expansion of Turkey’s Medical Tourism Industry.  Voices in Bioethics ,  8 . https://doi.org/10.52214/vib.v8i.9894

[76] Stem Cell Tourism: False Hope for Real Money . Harvard Stem Cell Institute (HSCI). (2023). https://hsci.harvard.edu/stem-cell-tourism , See also: Bissassar, M. (2017). Transnational Stem Cell Tourism: An ethical analysis.  Voices in Bioethics ,  3 . https://doi.org/10.7916/vib.v3i.6027

[77] Song, P. (2011) The proliferation of stem cell therapies in post-Mao China: problematizing ethical regulation,  New Genetics and Society , 30:2, 141-153, DOI:  10.1080/14636778.2011.574375

[78] Dajani, R. (2014). Jordan’s stem-cell law can guide the Middle East.  Nature  510, 189. https://doi.org/10.1038/510189a

[79] International Society for Stem Cell Research. (2024). Standards in stem cell research . International Society for Stem Cell Research. https://www.isscr.org/guidelines/5-standards-in-stem-cell-research

[80] Benjamin, R. (2013). People’s science bodies and rights on the Stem Cell Frontier . Stanford University Press.

Mifrah Hayath

SM Candidate Harvard Medical School, MS Biotechnology Johns Hopkins University

Olivia Bowers

MS Bioethics Columbia University (Disclosure: affiliated with Voices in Bioethics)

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NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD)

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Effect of Stool Sampling on a Routine Clinical Method for the Quantification of Six Short Chain Fatty Acids in Stool Using Gas Chromatography-Mass Spectrometry.

Author(s): Mahdi T, Desmons A, Krasniqi P, Lacorte JM, Kapel N, Lamazière A, Fourati S, Eguether T

Publication: Microorganisms , 2024, Vol. 12 , Page

PubMed ID: 38674773 PubMed Review Paper? No

Purpose of Paper

This paper compared the levels of six short-chain fatty acids (SCFAs) in matched fecal specimens that were stored at room temperature for up to 8 h; diluted in ethanol, methanol, or RNAlater; lyophilized, lyophilized and diluted in glycerol, or analyzed fresh; lyophilized or not and freeze-thaw cycled up to two times; lyophilized and stored at 4°C for up to 130 days; or stored after extraction and derivatization at room temperature for up to 7 days. Levels of the six SCFAs were also compared in feces from healthy volunteers and patients with irritable bowel disease. 

Conclusion of Paper

Storage of feces at room temperature resulted in declines in each of the six SCFAs analyzed during the first 2 h, but levels subsequently increased such that after 6 h they were more similar to specimens that were immediately frozen. Concentrations of each of the six SCFAs were lower when diluted with ethanol, methanol or RNAlater than in matched unpreserved specimens. Compared to fresh feces, lyophilized feces had higher levels of each of the six SCFAs, but levels were comparable to fresh feces when 10% glycerol was added to lyophilized samples. Freeze-thaw cycling affected the levels of all six SCFAs, but the magnitude of effects was larger in non-lyophilized than in lyophilized specimens. Changes in the levels of all six SCFAs were limited to <15% in at least two of the three lyophilized specimens that were stored for ≤60 days at 4°C.  Levels of the six SCFAs changed by <15% when the extract from lyophilized or fresh feces was stored for 7 days at room temperature. Levels of the six SCFAs in feces from patients the IBD cohort fell within the range of those found in feces from healthy patients.    

Study Purpose

This study compared levels of six short-chain fatty acids (SCFAs) in matched fecal specimens that were stored at room temperature for up to 8 h; diluted in ethanol, methanol, or RNAlater; lyophilized, lyophilized and diluted in glycerol, or analyzed fresh; lyophilized or not and freeze-thaw cycled up to two times; lyophilized and stored at 4°C for up to 130 days; or stored after extraction and derivatization at room temperature for up to 7 days. Levels of the six SCFAs were also compared in feces from healthy volunteers and patients with irritable bowel disease.  Fecal specimens were collected from twenty-eight healthy volunteers (20-65 years of age) and sent to the laboratory within 2 h.  Fecal specimens were either aliquoted and lyophilized or stored at 4°C or -80°C. Lyophilized specimens were frozen at -80°C for >24 h before lyophilization using a Lyovapor Buchi L-200. SCFA were extracted and derivatized using isobutyl chloroformiate and quantified by GS-MS. To evaluate effects of preservation method, aliquots of a single fecal specimen were left undiluted (fresh) or diluted in ethanol, methanol, or RNAlater. To evaluate SCFA stability at room temperature, a single stool specimen was stored at room temperature, and aliquots were frozen at -80°C after 0, 2, 4, 6, and 8 h. To investigate potential effects of freeze-thaw cycling, a fecal specimen was aliquoted and analyzed fresh and after lyophilization and storage at -80°C or after two or five freeze-thaw cycles. To evaluate effects associated with storage of lyophilized feces at 4°C, three lyophilized fecal specimens were analyzed after 1, 12, 33, 60, and 130 days at 4°C. To analyze effects of including 10% glycerol as a cryoprotectant, three fecal specimens were diluted in 10% glycerol and compared to fresh and lyophilized specimens. To investigate the stability of extracts, specimens were extracted, derivatized, and analyzed after 0 and 7 days at 4°C and in ten specimens after 0 and 24 h at room temperature. Levels of the six SCFAs were compared in feces from the twenty-eight healthy volunteers and twenty-seven patients with irritable bowel diseases (four patients with ulcerative colitis and twenty-three patients with Crohn’s disease) who were undergoing fecal calprotectin analysis.

Summary of Findings:

The assay had an inter- and intra- day precision standard deviation of 3-10% and 1-5%, respectively, for all six SCFAs evaluated. Recovery of the six SCFAs from spiked specimens generally ranged from 80-120% but was 129% for isovalerate, which the authors state indicates a risk of overestimation. After correction, concentrations of each of the six SCFAs were less than 20% those in matched fresh specimens when diluted with ethanol or RNAlater. In contrast, when feces were diluted in methanol, concentrations of the six SCFAs were 44-68% of those in matched fresh specimens. Storage of feces at room temperature resulted in declines of 11-29% for each of the six SCFAs after only 2 h, but levels then increased such that after 6 h they were more similar to specimens that were immediately frozen; the authors state that SCFAs levels were also acceptable in feces after 8 h of storage at room temperature. Compared to fresh feces, lyophilized feces had higher levels of each of the six SCFAs, but when 10% glycerol was added to lyophilized feces, the levels were comparable to fresh feces. When lyophilized specimens were freeze-thaw cycled twice, isobutyrate and valerate declined by 32 and 35%, respectively, but the other 4 SCFAs declined by less than 10%. However, after five freeze-thaw cycles, acetate, propionate, and butyrate decreased by 10-30% and isovalerate, isobutyrate, and valerate decreased by 48-50%. Freeze-thaw cycling had a larger effect on non-lyophilized feces, with 15-40% declines in all six SCFAs after only two freeze-thaw cycles. Levels of acetate, propionate, and butyrate in lyophilized feces declined by <15% after 60 days at 4°C, and levels of isobutyrate, isovalerate, and valerate also declined by <15% in two of the three lyophilized specimens evaluated after 60 days of storage. Storage of lyophilized feces at 4°C for 130 days led to declines >15% in five of the six SCFAs; however, changes in butyrate were limited to 2-12%.  Levels of the 6 SCFAs changed by -14 to 3% after the extract from lyophilized or fresh feces was stored for 7 days at room temperature. Levels of the six SCFAs in feces from patients the IBD cohort fell within the range of those found in feces from healthy patients.  

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Biospecimens

  • Bodily Fluid - Feces

Preservative Types

  • Other Preservative
  • None (Fresh)
  • Irritable Bowel Syndrome

Pre-analytical Factors:

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Facility for Rare Isotope Beams

At michigan state university, international research team uses wavefunction matching to solve quantum many-body problems, new approach makes calculations with realistic interactions possible.

FRIB researchers are part of an international research team solving challenging computational problems in quantum physics using a new method called wavefunction matching. The new approach has applications to fields such as nuclear physics, where it is enabling theoretical calculations of atomic nuclei that were previously not possible. The details are published in Nature (“Wavefunction matching for solving quantum many-body problems”) .

Ab initio methods and their computational challenges

An ab initio method describes a complex system by starting from a description of its elementary components and their interactions. For the case of nuclear physics, the elementary components are protons and neutrons. Some key questions that ab initio calculations can help address are the binding energies and properties of atomic nuclei not yet observed and linking nuclear structure to the underlying interactions among protons and neutrons.

Yet, some ab initio methods struggle to produce reliable calculations for systems with complex interactions. One such method is quantum Monte Carlo simulations. In quantum Monte Carlo simulations, quantities are computed using random or stochastic processes. While quantum Monte Carlo simulations can be efficient and powerful, they have a significant weakness: the sign problem. The sign problem develops when positive and negative weight contributions cancel each other out. This cancellation results in inaccurate final predictions. It is often the case that quantum Monte Carlo simulations can be performed for an approximate or simplified interaction, but the corresponding simulations for realistic interactions produce severe sign problems and are therefore not possible.

Using ‘plastic surgery’ to make calculations possible

The new wavefunction-matching approach is designed to solve such computational problems. The research team—from Gaziantep Islam Science and Technology University in Turkey; University of Bonn, Ruhr University Bochum, and Forschungszentrum Jülich in Germany; Institute for Basic Science in South Korea; South China Normal University, Sun Yat-Sen University, and Graduate School of China Academy of Engineering Physics in China; Tbilisi State University in Georgia; CEA Paris-Saclay and Université Paris-Saclay in France; and Mississippi State University and the Facility for Rare Isotope Beams (FRIB) at Michigan State University (MSU)—includes  Dean Lee , professor of physics at FRIB and in MSU’s Department of Physics and Astronomy and head of the Theoretical Nuclear Science department at FRIB, and  Yuan-Zhuo Ma , postdoctoral research associate at FRIB.

“We are often faced with the situation that we can perform calculations using a simple approximate interaction, but realistic high-fidelity interactions cause severe computational problems,” said Lee. “Wavefunction matching solves this problem by doing plastic surgery. It removes the short-distance part of the high-fidelity interaction, and replaces it with the short-distance part of an easily computable interaction.”

This transformation is done in a way that preserves all of the important properties of the original realistic interaction. Since the new wavefunctions look similar to that of the easily computable interaction, researchers can now perform calculations using the easily computable interaction and apply a standard procedure for handling small corrections called perturbation theory.  A team effort

The research team applied this new method to lattice quantum Monte Carlo simulations for light nuclei, medium-mass nuclei, neutron matter, and nuclear matter. Using precise ab initio calculations, the results closely matched real-world data on nuclear properties such as size, structure, and binding energies. Calculations that were once impossible due to the sign problem can now be performed using wavefunction matching.

“It is a fantastic project and an excellent opportunity to work with the brightest nuclear scientist s in FRIB and around the globe,” said Ma. “As a theorist , I'm also very excited about programming and conducting research on the world's most powerful exascale supercomputers, such as Frontier , which allows us to implement wavefunction matching to explore the mysteries of nuclear physics.”

While the research team focused solely on quantum Monte Carlo simulations, wavefunction matching should be useful for many different ab initio approaches, including both classical and  quantum computing calculations. The researchers at FRIB worked with collaborators at institutions in China, France, Germany, South Korea, Turkey, and United States.

“The work is the culmination of effort over many years to handle the computational problems associated with realistic high-fidelity nuclear interactions,” said Lee. “It is very satisfying to see that the computational problems are cleanly resolved with this new approach. We are grateful to all of the collaboration members who contributed to this project, in particular, the lead author, Serdar Elhatisari.”

This material is based upon work supported by the U.S. Department of Energy, the U.S. National Science Foundation, the German Research Foundation, the National Natural Science Foundation of China, the Chinese Academy of Sciences President’s International Fellowship Initiative, Volkswagen Stiftung, the European Research Council, the Scientific and Technological Research Council of Turkey, the National Natural Science Foundation of China, the National Security Academic Fund, the Rare Isotope Science Project of the Institute for Basic Science, the National Research Foundation of Korea, the Institute for Basic Science, and the Espace de Structure et de réactions Nucléaires Théorique.

Michigan State University operates the Facility for Rare Isotope Beams (FRIB) as a user facility for the U.S. Department of Energy Office of Science (DOE-SC), supporting the mission of the DOE-SC Office of Nuclear Physics. Hosting what is designed to be the most powerful heavy-ion accelerator, FRIB enables scientists to make discoveries about the properties of rare isotopes in order to better understand the physics of nuclei, nuclear astrophysics, fundamental interactions, and applications for society, including in medicine, homeland security, and industry.

The U.S. Department of Energy Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of today’s most pressing challenges. For more information, visit energy.gov/science.

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